MicroRNA-21 promotes glioma cell proliferation and inhibits senescence and apoptosis by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway (Retracted Article)
MicroRNA-21 promotes glioma cell proliferation and inhibits senescence and apoptosis by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway (Retracted Article)
复制标题
MicroRNA-21通过PTEN/PI3K/AKT信号通路靶向SPRY1促进胶质瘤细胞增殖，抑制衰老和凋亡
DOI：
10.1111/cns.12785
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发表时间：
2018-05-01
影响因子：
5.5
通讯作者：
Li, Ming
中科院分区：
文献类型：
--
作者：
Chai, Chang;Song, Lai-Jun;Li, Ming
目的探讨microRNA-21（miR-21）通过PTEN/PI 3 K/AKT信号通路对胶质瘤细胞增殖、衰老和凋亡的影响。采用RT-qPCR测量miR-21、SPRY 1、PTEN、PI 3 K和AKT的mRNA水平，并进行蛋白质印迹以确定SPRY 1、PTEN、PI 3 K、AKT、p-AKT、Caspase-3、Caspase-9、P53、GSK 3和p-GSK 3的蛋白质水平。将人胶质瘤U87细胞分为空白组、阴性对照组（NC）、miR-21模拟物组、miR-21抑制剂组、siRNA-SPRY 1组和miR-21抑制剂+siRNA-SPRY 1组，人HEB细胞作为正常组。结果与对照组相比，胶质瘤组miR-21、PI 3 K、AKT、p-AKT、P53、p-GSK 3表达增加，SPRY 1、PTEN、Caspase-3、Caspase-9表达减少，而GSK 3表达无明显差异。SPRY 1是miR-21的靶基因。与空白组和NC组相比，miR-21 mimics组和siRNA-SPRY 1组PI 3 K、AKT、p-AKT、P53和p-GSK 3水平升高，SPRY 1、PTEN、Caspase-3和Caspase-9水平降低，miR-21抑制剂组则相反;此外，miR-21抑制剂组显示细胞增殖降低但促进凋亡，结论MicroRNA-21可能通过PTEN/PI 3 K/AKT信号通路靶向SPRY 1，促进人脑胶质瘤细胞增殖，抑制细胞衰老和凋亡。
AimsOur study aims to investigate the effect of microRNA-21 (miR-21) on the proliferation, senescence, and apoptosis of glioma cells by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway.MethodsGlioma tissues and brain tissues were collected for this study after surgical decompression for traumatic brain injury. RT-qPCR was employed to measure mRNA levels of miR-21, SPRY1, PTEN, PI3K, and AKT, and Western blotting was conducted to determine protein levels of SPRY1, PTEN, PI3K, AKT, p-AKT, Caspase-3, Caspase-9, P53, GSK3, and p-GSK3. Human glioma U87 cells were assigned into the blank, negative control (NC), miR-21 mimics, miR-21 inhibitors, siRNA-SPRY1, and miR-21 inhibitors + siRNA-SPRY1 groups, with human HEB cells serving as the normal group. Cell proliferation, cell cycle, and apoptosis were determined by MTT and flow cytometry, respectively.ResultsCompared with control group, an increased expression of miR-21, PI3K, AKT, p-AKT, P53, and p-GSK3, and a decreased expression of SPRY1, PTEN, Caspase-3, and Caspase-9 were observed in the glioma group, and no significant differences were found in the expression of GSK3. SPRY1 was verified to be the target gene of miR-21. Compared with the blank and NC groups, levels of PI3K, AKT, p-AKT, P53, and p-GSK3 increased while levels of SPRY1, PTEN, Caspase-3, and Caspase-9 decreased in the miR-21 mimics and siRNA-SPRY1 groups; the miR-21 inhibitors group reversed the tendency; furthermore, the miR-21 inhibitors group showed decreased cell proliferation but promoted apoptosis, which were opposite to the results of the miR-21 mimics and siRNA-SPRY1 groups.ConclusionMicroRNA-21 might promote cell proliferation and inhibit cell senescence and apoptosis of human glioma cells by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway.