Studies of consomic mice bearing the Y chromosome of the BXSB mouse.
Studies of consomic mice bearing the Y chromosome of the BXSB mouse.
复制标题
对携带 BXSB 小鼠 Y 染色体的体鼠的研究。
作者：
C. C. Hudgins;R. T. Steinberg;D. Klinman;M. J. Reeves;A. Steinberg
先前的研究表明，BXSB小鼠的Y染色体可导致近交系BXSB小鼠和F1杂交种的自身免疫加速。为了进一步研究BXSB-Y的作用，我们研究了三组y -经济小鼠，NZB。BXSB-Y NZW。BXSB-Y和CBA/J。BXSB-Y分别由来自非BXSB亲本的背景基因和来自BXSB小鼠的Y染色体组成。评估BXSB-Y对自身抗体产生、免疫病理和生存的影响。我们发现CBA/J。BXSB-Y小鼠与对照CBA/J雄性小鼠差异不大。相比之下，纽元。BXSB- y雄性小鼠的肾脏和心脏疾病和早期死亡加速，类似于先前报道的（NZW X BXSB）F1小鼠。NZB。BXSB-Y男性抗红细胞自身抗体加速，但抗dna不加速。它们的寿命几乎和NZB小鼠一样长。BXSB- y在所有经济小鼠中都存在，通过与BXSB雌性经济小鼠杂交证实，并且在雄性后代中显示加速疾病。本研究表明，BXSB-Y染色体自身免疫加速因子不是单独起作用，而是通过其他基因起作用，并且对不同遗传背景的影响是不同的。这些研究对人类狼疮有启示；它们也为BXSB-Y及其作用基因的未来分子生物学研究提供了基础。
Previous studies have demonstrated that the Y chromosome of the BXSB mouse can lead to accelerated autoimmunity in inbred BXSB mice and in F1 hybrids. To additionally study the effects of the BXSB-Y, we have studied three sets of Y-consomic mice, NZB.BXSB-Y, NZW.BXSB-Y, and CBA/J.BXSB-Y, each consisting of background genes from the non-BXSB parent and the Y chromosome from the BXSB mouse. The effect of the BXSB-Y on autoantibody production, immunopathology, and survival was assessed. We found that the CBA/J.BXSB-Y mice showed few differences from control CBA/J males. In contrast, NZW.BXSB-Y males had accelerated renal and cardiac disease and early death, resembling that previously reported for (NZW X BXSB)F1 mice. NZB.BXSB-Y males had accelerated anti-erythrocyte autoantibodies but not accelerated anti-DNA. They lived almost as long as NZB mice. The presence of the BXSB-Y in all of the consomic mice was confirmed by crossing the consomic mice with BXSB females and demonstrating accelerated disease in the male offspring. This study demonstrates that the BXSB-Y chromosome autoimmune accelerating factor does not act alone but operates through other genes, and that the effects on different genetic backgrounds are different. The studies have implications for human lupus; they also provide a basis for future molecular biology studies of the BXSB-Y and the genes upon which it acts.