Use of recA as an alternative phylogenetic marker in the family Vibrionaceae
Use of recA as an alternative phylogenetic marker in the family Vibrionaceae
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DOI：
10.1099/ijs.0.02963-0
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Swings, J
中科院分区：
文献类型：
--
作者：
Thompson, CC;Thompson, FL;Swings, J
这项研究分析了recA基因序列作为弧菌的替代系统发育和/或鉴定标记的有用性。RecA序列表明弧菌属为多系群。在弧菌中观察到的高度异质性与以前对这一组的多相分类研究是一致的。发光菌聚集在一起，并明显嵌套在弧菌中，而Grimontia hollisae与其他弧菌是分开的。在弧菌中，霍乱弧菌和拟态弧菌与其他属成员分开聚集。哈维氏弧菌和与华丽弧菌有亲缘关系的物种组成了紧密的分离群。另一方面，与途氏弧菌相关的物种在系统发育树上出现零星分布。珊瑚弧菌和尼伯氏弧菌、内氏弧菌和XUII弧菌、图氏弧菌和巴西弧菌完全分开聚在一起。RecA与16S rDNA成对相似性相关系数为0.58。同一物种的菌株具有至少94%的recA序列相似性。RecA基因序列比16S rDNA具有更大的区分性。对于高于98%的16S rDNA相似值，recA的相似性范围很大，从83%到99%。
This study analysed the usefulness of recA gene sequences as an alternative phylogenetic and/or identification marker for vibrios. The recA sequences suggest that the genus Vibrio is polyphyletic. The high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. Photobacterium species clustered together and apparently nested within vibrios, while Grimontia hollisae was apart from other vibrios. Within the vibrios, Vibrio cholerae and Vibrio mimicus clustered apart from the other genus members. Vibrio harveyi- and Vibrio splendidus-related species formed compact separated groups. On the other hand, species related to Vibrio tubiashii appeared scattered in the phylogenetic tree. The pairs Vibrio coralliilyticus and Vibrio neptunius, Vibrio nereis and Vibrio xuii and V. tubiashii and Vibrio brasiliensis clustered completely apart from each other. There was a correlation of 0.58 between recA and 16S rDNA pairwise similarities. Strains of the same species have at least 94% recA sequence similarity. recA gene sequences are much more discriminatory than 16S rDNA. For 16S rDNA similarity values above 98% there was a wide range of recA similarities, from 83 to 99%.