Replication characteristics of swine influenza viruses in precision-cut lung slices reflect the virulence properties of the viruses
Replication characteristics of swine influenza viruses in precision-cut lung slices reflect the virulence properties of the viruses
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DOI：
10.1186/1297-9716-44-110
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发表时间：
2013-11-13
影响因子：
4.4
通讯作者：
Herrler, Georg
中科院分区：
文献类型：
--
作者：
Meng, Fandan;Punyadarsaniya, Darsaniya;Herrler, Georg
精密切割的猪肺切片感染了五种不同亚型的甲型猪流感病毒（A/sw/Potsdam/15/1981 H1N1、A/sw/Bad Griesbach/IDT5604/2006 H1N1、A/sw/Bakum/1832/2000 H1N2、A/sw/Damme/IDT5673/2006 H3N2，A/sw/Herford/IDT5932/2007 H3N2)。这些病毒能够感染纤毛细胞和产生粘液的细胞。分析了猪甲型流感病毒对分化良好的呼吸道上皮细胞的感染，分析了病毒释放到上清液中的动力学。 H3N2/2006 和 H3N2/2007 病毒的滴度最高。 H1N1/1981 和 H1N2/2000 病毒的复制速度比 H3N2 病毒稍慢，而 2006 年的 H1N1 病毒株的繁殖滴度明显低于其他病毒株。就其诱导纤毛抑制效应的能力而言，这两种 H3N2 病毒株的毒性最强。 H1N1/1981 和 H1N2/2000 对纤毛活动的影响稍弱一些。 H1N1/2006 观察到的纤毛抑制效果最低。为了研究这一发现是否与宿主中相应的毒力相关，用 H3N2/2006、H1N2/2000、H1N1/1981 和 H1N1/2006 病毒实验性感染猪。 H1N1/2006 病毒对猪的毒力明显低于其他病毒，这与体外研究的结果一致。这些发现为开发能够评估猪甲型流感病毒毒力的离体系统提供了可能性。
Precision-cut lung slices of pigs were infected with five swine influenza A viruses of different subtypes (A/sw/Potsdam/15/1981 H1N1, A/sw/Bad Griesbach/IDT5604/2006 H1N1, A/sw/Bakum/1832/2000 H1N2, A/sw/Damme/IDT5673/2006 H3N2, A/sw/Herford/IDT5932/2007 H3N2). The viruses were able to infect ciliated and mucus-producing cells. The infection of well-differentiated respiratory epithelial cells by swine influenza A viruses was analyzed with respect to the kinetics of virus release into the supernatant. The highest titres were determined for H3N2/2006 and H3N2/2007 viruses. H1N1/1981 and H1N2/2000 viruses replicated somewhat slower than the H3N2 viruses whereas a H1N1 strain from 2006 multiplied at significantly lower titres than the other strains. Regarding their ability to induce a ciliostatic effect, the two H3N2 strains were found to be most virulent. H1N1/1981 and H1N2/2000 were somewhat less virulent with respect to their effect on ciliary activity. The lowest ciliostatic effect was observed with H1N1/2006. In order to investigate whether this finding is associated with a corresponding virulence in the host, pigs were infected experimentally with H3N2/2006, H1N2/2000, H1N1/1981 and H1N1/2006 viruses. The H1N1/2006 virus was significantly less virulent than the other viruses in pigs which was in agreement with the results obtained by the in vitro-studies. These findings offer the possibility to develop an ex vivo-system that is able to assess virulence of swine influenza A viruses.