Simultaneous determination of benzodiazepines and their metabolites in human serum by liquid chromatography-tandem mass spectrometry using a high-resolution octadecyl silica column compatible with aqueous compounds
Simultaneous determination of benzodiazepines and their metabolites in human serum by liquid chromatography-tandem mass spectrometry using a high-resolution octadecyl silica column compatible with aqueous compounds
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DOI：
10.1002/bmc.1122
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发表时间：
2009-04-01
影响因子：
1.8
通讯作者：
Hirano, Kazuyuki
中科院分区：
文献类型：
--
作者：
Nakamura, Mitsuhiro;Ohmori, Tomofumi;Hirano, Kazuyuki
建立了液相色谱-串联质谱法（LC-MS/MS）同时测定人血清中苯二氮卓类药物及其代谢物的方法。该方法能够同时测定多种苯二氮卓类药物，包括泮、溴西泮、利血平、硝西泮、氯硝西泮、氟硝西泮、艾司唑仑、氯巴占、劳拉西泮、阿普唑仑、三唑仑、溴替唑仑、氟地西泮、地西泮、夸西泮、普拉西泮及其代谢产物7-氨基硝西泮、7-氨基氯硝西泮、7-乙酰胺硝西泮、N-去甲基氯巴占和N-去甲基地西泮。将掺入人血清中的分析物进行固相萃取，然后进行液相色谱-电喷雾串联质谱联用。22种苯二氮卓类药物及其代谢产物的检测时间在25 min以内。除夸西泮的回收率为45.8%外，其余化合物的回收率均超过58.1%。检测限范围为0.3 - 11.4 ng/mL。所有化合物的线性均符合要求。这些数据表明，本方法可适用于常规测定苯二氮卓类药物在临床设置。版权所有（C）2008约翰威利父子有限公司
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using a high-resolution octadecyl silica column compatible with aqueous compounds was developed for the simultaneous determination of benzodiazepines and their metabolites in human serum. This method enabled us to determine multiple benzodiazepines, Including flurazepam, bromazepam, chlordiazepoxide, nitrazepam, clonazepam, flunitrazepam, estazolam, clobazam, lorazepam, alprazolam, triazolam, brotizolam, fludiazepam, diazepam, quazepam, prazepam and their metabolites such as 7-aminonitrazepam, 7-aminoclonazepam, 7-acetamidonitrazepam, N-desmethylclobazam and N-desmethyidiazepam. The analytes spiked into human serum were subjected to solid-phase extraction followed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry. The running time was within 25 min for the measurement of 22 benzodiazepines and their metabolites. The recovery rates exceeded 58.1% for those compounds except for quazepam, which showed a recovery of 45.8%. The limit of detection ranged from 0.3 to 11.4 ng/mL. Linearity was satisfactory for all compounds. These data suggest that the present method can be applicable to routine assay for benzodiazepines In the clinical setting. Copyright (C) 2008 John Wiley & Sons, Ltd.