MOLECULAR-CLONING AND EXPRESSION OF HUMAN HEPATOCYTE GROWTH-FACTOR
MOLECULAR-CLONING AND EXPRESSION OF HUMAN HEPATOCYTE GROWTH-FACTOR
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DOI：
10.1038/342440a0
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发表时间：
1989-11-23
期刊：
影响因子：
64.8
通讯作者：
SHIMIZU, S
中科院分区：
文献类型：
--
作者：
NAKAMURA, T;NISHIZAWA, T;SHIMIZU, S
肝细胞生长因子（HGF）是成熟薄壁组织中最有效的丝裂原！原代培养中的肝细胞，似乎是一种肝营养因子，在部分肝切除术和肝损伤后作为肝脏再生的触发因素。HGF的部分纯化和表征已有报道1 - 4。我们已经证明，来自大鼠血小板的纯HGF是一种新的生长因子，6在低至1 ng mP−1的浓度下有效。HGF和表皮生长因子（EGF）的作用是叠加的。HGF的活性不是物种特异性的，尽管它不刺激Swiss 3T3成纤维细胞的生长5。HGF的相对分子质量（Mr）为82,000，是由mr69,000的大a亚基和mr34,000的小β亚基组成的异源二聚体（参考文献6）。本文报道了利用互补DNA克隆测定人HGF的氨基酸序列，并在转染克隆cDNA的COS-1细胞中表达具有生物活性的人HGF。人类HGF cDNA的核苷酸序列显示，α-链和β-链都包含在一个开放阅读框中，编码728个氨基酸的前前体蛋白。
HEPATOCYTE growth factor (HGF) is the most potent mitogen for mature parenchyma! hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification and characterization of HGF have been reported1–4. We have demonstrated that pure HGF from rat platelets is a new growth factor5, 6effective at concentrations as low as 1 ng mP−1.The effects of HGF and epidermal growth factor (EGF) are additive. The activity of HGF is not species-specific, although it does not stimulate growth in Swiss 3T3 fibroblasts5. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large a-subunit ofMr69,000 and a smallβ-subunit ofMr34,000 (ref. 6). Here we report the amino-acid sequence of human HGF determined by complementary DNA cloning and the expression of biologically active human HGF from COS-1 cells transfected with cloned cDNA. The nucleotide sequence of the human HGF cDNA reveals that bothα- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids.