ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD
ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD
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DOI：
10.1126/science.1312257
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发表时间：
1992-02-28
期刊：
影响因子：
56.9
通讯作者：
ZINK, JI
中科院分区：
文献类型：
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作者：
ELLERBY, LM;NISHIDA, CR;ZINK, JI
在温和的条件下，采用新型溶胶-凝胶合成技术将铜锌超氧化物歧化酶（CuZnSOD）、细胞色素c和肌红蛋白（Mb）包裹在稳定的、光学透明的多孔硅玻璃基质中，使生物分子保持其特有的反应活性和光谱特性。由此产生的玻璃允许小分子以合理的速率进出玻璃，但仍将蛋白质分子保留在其孔内。固定化蛋白的化学反应可以通过其可见吸收光谱的变化来监测。观察到含有固定化蛋白质的硅玻璃具有与溶液中蛋白质相似的反应活性和光谱性质。例如，包覆CuZnSOD脱金属再金属化，包覆铁铬c还原再氧化，包覆的met Mb还原为脱氧Mb，然后与二氧反应生成氧Mb或与一氧化碳反应生成羰基Mb。
Novel sol-gel synthetic techniques were used to immobilize copper-zinc superoxide dismutase (CuZnSOD), cytochrome c, and myoglobin (Mb) by encapsulation in stable, optically transparent, porous silica glass matrices under mild conditions such that the biomolecules retained their characteristic reactivities and spectroscopic properties. The resulting glasses allowed transport of small molecules into and out of the glasses at reasonable rates but nevertheless retained the protein molecules within their pores. Chemical reactions of the immobilized proteins could be monitored by means of changes in their visible absorption spectra. Silica glasses containing the immobilized proteins were observed to have similar reactivities and spectroscopic properties to those found for the proteins in solution. For example, encapsulated CuZnSOD was demetallated and remetallated, encapsulated ferricytochrome c was reduced and then reoxidized, and encapsulated met Mb was reduced to deoxy Mb and then reacted either with dioxygen to make oxy Mb or with carbon monoxide to make carbonyl Mb.