Infection sites of Pseudomonas cichorii into head leaf of lettuce
Infection sites of Pseudomonas cichorii into head leaf of lettuce
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DOI：
10.3186/jjphytopath.62.125
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Suzuki, Kazumi
中科院分区：
文献类型：
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作者：
Hikichi, Yasufumi;Saito, Akira;Suzuki, Kazumi
用异硫氰酸荧光素标记的抗莴苣细菌性腐烂病病原体菊苣假单胞菌的抗体对莴苣中头叶切片进行染色，并在荧光显微镜下观察。切片的气孔中观察到荧光抗体标记的菊苣细胞，不存在病害的褐变症状。菊苣特有的荧光斑点主要分布在气孔保卫细胞和气孔下腔的细胞间隙中。褐变，这是疾病的最初症状，从表皮发展到叶肉。在褐变症状进展之前，在表皮细胞间隙中观察到特异性荧光斑点，然后在叶肉细胞间隙中观察到特异性荧光斑点。这些结果表明，菊苣疫霉首先通过气孔侵入莴苣头叶，然后在表皮细胞间隙繁殖，最后定殖在叶肉细胞间隙。在叶毛中也观察到特异的荧光斑点。
Sections of middle head leaf of lettuce were stained with fluorescein isothiocyanate-conjugated antibody against Pseudomonas cichorii, a causal agent of bacterial rot of lettuce, and were observed under a fluorescent microscope. Fluorescent antibody-labeled P. cichorii cells were observed in stomata of the sections, which did not contain browing symptom of the disease. Special fluorescent specks derived from P. cichorii were most frequently observed in guard cells and intercellular spaces of substomatal cavities. Browing, which was the initial symptom of disease, progressed from epidermis to mesophyll. In advance of progress of browing symptom, specific fluorescent specks were observed in intercellular spaces of epidermis and then were observed in intercellular spaces of mesophyll. These results suggested that P. cichorii first invaded head leaf of lettuce through stomata, later multiplied in intercellular spaces of epidermis and colonized intercellular spaces of mesophyll. Specific fluorescent specks were also observed in leaf hairs.