Simple and Versatile 3D Printed Microfluidics Using Fused Filament Fabrication.
Simple and Versatile 3D Printed Microfluidics Using Fused Filament Fabrication.
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DOI：
10.1371/journal.pone.0152023
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Castell OK
中科院分区：
文献类型：
--
作者：
Morgan AJ;Hidalgo San Jose L;Jamieson WD;Wymant JM;Song B;Stephens P;Barrow DA;Castell OK
更广泛的科学界对微流体的吸收受到了传统微流体设备生产所需的技能和设备所造成的制造障碍的限制。在这里，我们提出了简单的3D打印微流体设备使用廉价和易于访问的打印机与商业上可用的打印机材料。我们证明，以前报道的透明度和保真度的限制已被克服，而能够在超过2000千帕的压力下工作的设备说明泄漏问题也得到了解决。通过将牙髓干细胞封装在藻酸盐液滴中来说明3D打印微流体装置的实用性;细胞活力测定显示绝大多数细胞仍然存活，并且装置透明度足以用于单细胞成像。这些设备的可访问性通过集成端口的制造以及通过引入Lego®类模块化系统来进一步增强，该系统有助于快速原型制作，同时为新手提供从微流体组件数据库构建微流体系统的可能性。
The uptake of microfluidics by the wider scientific community has been limited by the fabrication barrier created by the skills and equipment required for the production of traditional microfluidic devices. Here we present simple 3D printed microfluidic devices using an inexpensive and readily accessible printer with commercially available printer materials. We demonstrate that previously reported limitations of transparency and fidelity have been overcome, whilst devices capable of operating at pressures in excess of 2000 kPa illustrate that leakage issues have also been resolved. The utility of the 3D printed microfluidic devices is illustrated by encapsulating dental pulp stem cells within alginate droplets; cell viability assays show the vast majority of cells remain live, and device transparency is sufficient for single cell imaging. The accessibility of these devices is further enhanced through fabrication of integrated ports and by the introduction of a Lego®-like modular system facilitating rapid prototyping whilst offering the potential for novices to build microfluidic systems from a database of microfluidic components.