Validation of a mouse conductance system to determine LV volume: comparison to echocardiography and crystals
Validation of a mouse conductance system to determine LV volume: comparison to echocardiography and crystals
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DOI：
10.1152/ajpheart.2000.279.4.h1698
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发表时间：
2000-10-01
影响因子：
4.8
通讯作者：
Freeman, GL
中科院分区：
文献类型：
--
作者：
Feldman, MD;Erikson, JM;Freeman, GL
由于转基因小鼠心脏体积小，心跳快，因此将左心室压力-容积分析应用于转基因小鼠以表征心脏表型存在问题。电导技术已经为鼠标小型化，可以解决这个问题。然而，这项技术尚未得到验证。因此，我们在18只CD-1小鼠中进行了超声心动图检查，然后同时进行了超声晶体、流量探头和电导研究。对原始电导体积进行不均匀电场（a）和平行电导（G（pi））校正，得到每搏输出量为14.1 +/- 3.7 μ l/次，舒张末期容积为20.8 +/- 6.5 μ l，收缩末期容积为9.0 +/- 5.8 μ l。平均电导容积与流量探头和超声心动图得出的结果没有差异，但与超声晶体不同。G（pi）测定为14.9 +/- 8.7 μ l。然而，高渗盐水改变了小鼠左心室的尺寸和压力。虽然G（pi）可以通过高渗盐水方法测定，但盐水改变了血液动力学，质疑其在小鼠中的有效性。尽管不同技术之间绝对体积的平均测量值可能相似，但个体值不相关。
The application of left ventricular pressure-volume analysis to transgenic mice to characterize the cardiac phenotype has been problematic due to the small size of the mouse heart and the rapid heartbeat. Conductance technology has been miniaturized for the mouse and can solve this problem. However, there has been no validation of this technique. Accordingly, we performed echocardiography followed by simultaneous ultrasonic crystals, flow probe, and conductance studies in 18 CD-1 mice. Raw conductance volumes were corrected for an inhomogenous electrical field (a) and parallel conductance (G(pi)) yielding a stroke volume of 14.1 +/- 3.7 mu l/ beat, end-diastolic volume of 20.8 +/- 6.5 mu l, and end-systolic volume of 9.0 +/- 5.8 mu l. The mean conductance volumes were no different from those derived by flow probe and echocardiography but did differ from ultrasonic crystals. G(pi) was determined to be 14.9 +/- 8.7 mu l. However, hypertonic saline altered dimension and pressure in the mouse left ventricle. Although G(pi) can be determined by the hypertonic saline method, saline altered hemodynamics, questioning its validity in the mouse. Although mean measures of absolute volume may be similar among different techniques, individual values did not correlate.