Active zone localization of presynaptic calcium channels encoded by the cacophony locus of Drosophila
Active zone localization of presynaptic calcium channels encoded by the cacophony locus of Drosophila
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DOI：
10.1523/jneurosci.3553-03.2004
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发表时间：
2004-01-07
影响因子：
5.3
通讯作者：
Ordway, RW
中科院分区：
文献类型：
--
作者：
Kawasaki, F;Zou, BY;Ordway, RW
突触前钙通道通过提供诱发神经递质释放的钙触发剂在化学突触传递中发挥核心作用。这些电压门控钙通道由主要结构亚基α 1以及辅助β和α 2 δ亚基组成。我们以前的遗传，分子和功能分析表明，杂音（cac）基因编码的初级突触前钙通道α 1亚基在果蝇。在这里，我们报告了转基因表达的cac编码的α 1亚基融合增强绿色荧光蛋白有效地挽救了cac致死突变，并允许在体内分析钙通道定位在活动区。本文报道的结果进一步表征了钙通道在神经递质释放中的主要作用。此外，这些研究提供了一个独特的遗传工具，活的功能突触前钙离子通道在体内成像，并定义了一个分子标记的其他突触前蛋白相对于活性区的免疫定位。这些发现有望促进对这一重要模型系统中突触发育和功能的进一步分析。
Presynaptic calcium channels play a central role in chemical synaptic transmission by providing the calcium trigger for evoked neurotransmitter release. These voltage-gated calcium channels are composed of a primary structural subunit, alpha1, as well as auxiliary beta and alpha2delta subunits. Our previous genetic, molecular, and functional analysis has shown that the cacophony (cac) gene encodes a primary presynaptic calcium channel alpha1 subunit in Drosophila. Here we report that transgenic expression of a cac-encoded alpha1 subunit fused with enhanced green fluorescent protein efficiently rescues cac lethal mutations and allows in vivo analysis of calcium channel localization at active zones. The results reported here further characterize the primary role of cac-encoded calcium channels in neurotransmitter release. In addition, these studies provide a unique genetic tool for live imaging of functional presynaptic calcium channels in vivo and define a molecular marker for immunolocalization of other presynaptic proteins relative to active zones. These findings are expected to facilitate additional analysis of synaptic development and function in this important model system.