Engineered microenvironment for the study of myofibroblast mechanobiology.
Engineered microenvironment for the study of myofibroblast mechanobiology.
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DOI：
10.1111/wrr.12955
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Zhao R
中科院分区：
文献类型：
--
作者：
Xu Y;Koya R;Ask K;Zhao R
肌成纤维细胞是机械敏感细胞，其分化、迁移、作用力和生物合成等多种行为都受到周围微环境的调控。工程细胞培养模型被用来研究微环境因素如基质硬度、细胞外基质(ECM)的形态和应变以及剪应力对肌成纤维细胞生物学的影响。这些工程模型提供了模拟良好的、病理生理学相关的实验条件，优于传统的2D培养模型。从这个角度出发，我们将综述肌成纤维细胞工程化细胞培养模型的最新进展，并概述在不同微环境条件下肌成纤维细胞机制生物学的研究结果。这些研究已经证明了工程模型在研究微环境调节的细胞行为方面的威力和实用性。使用这些模型得出的发现有助于更好地理解肌成纤维细胞行为是如何在组织修复和病理性瘢痕形成中受到调节的。
Myofibroblasts are mechanosensitive cells and a variety of their behaviors including differentiation, migration, force production and biosynthesis are regulated by the surrounding microenvironment. Engineered cell culture models have been developed to examine the effect of microenvironmental factors such as the substrate stiffness, the topography and strain of the extracellular matrix (ECM) and the shear stress on myofibroblast biology. These engineered models provide well-mimicked, pathophysiologically-relavent experimental conditions that are superior to those enabled by the conventional 2D culture models. In this perspective, we will review the recent advances in the development of engineered cell culture models for myofibroblasts and outline the findings on the myofibroblast mechanobiology under various microenvironmental conditions. These studies have demonstrated the power and utility of the engineered models for the study of microenvironment-regulated cellular behaviors. The findings derived using these models contribute to a greater understanding of how myofibroblast behavior is regulated in tissue repair and pathological scar formation.
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DOI：
10.1164/rccm.201903-0497pp
发表时间：
2019-07-15
影响因子：
24.7
作者：
Albert, Richard K.;Smith, Bradford;Schwartz, David A.
通讯作者：
Schwartz, David A.
DOI：
10.1073/pnas.1611324113
发表时间：
2016-10-04
影响因子：
11.1
作者：
Hartman, Christopher D.;Isenberg, Brett C.;Wong, Joyce Y.
通讯作者：
Wong, Joyce Y.
影响因子：
3.7
作者：
Hartman CD;Isenberg BC;Chua SG;Wong JY
通讯作者：
Wong JY
影响因子：
4.6
作者：
Chen Z;Wang Q;Asmani M;Li Y;Liu C;Li C;Lippmann JM;Wu Y;Zhao R
通讯作者：
Zhao R
细胞介导的纤维募集驱动工程原纤维微环境中的细胞外基质机械感应。
影响因子：
41.2
作者：
Baker BM;Trappmann B;Wang WY;Sakar MS;Kim IL;Shenoy VB;Burdick JA;Chen CS
通讯作者：
Chen CS