Matrix metalloproteinase (MMP) expression in experimental choroidal neovascularization
Matrix metalloproteinase (MMP) expression in experimental choroidal neovascularization
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DOI：
10.1076/0271-3683(200009)2131-rft684
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发表时间：
2000-01
影响因子：
2
通讯作者：
A. Kvanta;W. Shen;S. Sarman;Stefan Seregard;B. Stéen;Elisabeth Rakoczy
中科院分区：
文献类型：
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作者：
A. Kvanta;W. Shen;S. Sarman;Stefan Seregard;B. Stéen;Elisabeth Rakoczy
目的。基质金属蛋白酶 (MMP) 是降解基底膜和细胞外基质蛋白的蛋白水解酶家族。为了获得有关 MMP 在脉络膜新生血管 (CNV) 中可能发挥的作用的信息，我们在大鼠模型中分析了 MMP-2 和 MMP-9（与眼部新生血管形成有关的两种形式的 MMP）的 mRNA 表达。方法。通过氪激光眼底光凝法在有色大鼠中诱导脉络膜新生血管形成，然后在第1、3、5、7、10和60天摘除眼睛。使用与 MMP-2 和 MMP-9 互补的 DNA 生成反义和正义核糖核酸探针，并使用原位杂交分析 mRNA 表达。结果。在未治疗的眼睛中，在脉络膜内的细胞中检测到微弱的 MMP-2 mRNA 表达。在激光治疗的眼睛中，MMP-2 mRNA 表达显着增加，主要位于侵入脉络膜、视网膜下腔和视网膜内层的巨噬细胞样和视网膜色素上皮 (RPE) 样细胞。 MMP-2 mRNA 表达的增加在第 10 天达到峰值，此后检测到下降。 MMP-9 mRNA 表达在未治疗的眼睛中较低，并且在激光治疗后没有增加。结论。结果表明，实验性 CNV 中 MMP-2 mRNA 表达增加，支持 MMP-2 在年龄相关性黄斑变性 CNV 发展中的作用。
Purpose. Matrix metalloproteinases (MMP) are a family of proteolytic enzymes that degrade basement membrane and extracellular matrix proteins. To gain information on the possible role of MMPs in choroidal neovascularization (CNV), we have analyzed the mRNA expression of MMP-2 and MMP-9, two forms of MMPs implicated in ocular neovascularization, in a rat model. Methods. Choroidal neovascularization was induced in pigmented rats by krypton laser photocoagulation of the fundus whereafter eyes were enucleated at 1, 3, 5, 7, 10 and 60 days. Antisense and sense riboprobes were generated using DNA complementary to MMP-2 and MMP-9, and mRNA expression was analyzed using in situ hybridization. Results. In the untreated eyes MMP-2 mRNA expression was weakly detected in cells within the choroid. In laser-treated eyes MMP-2 mRNA expression was markedly increased and mainly localized to macrophage-like and retinal pigment epithelial (RPE)-like cells invading the choroid, subretinal space and inner retina. This increase in MMP-2 mRNA expression peaked at day 10 whereafter a decline was detected. MMP-9 mRNA expression was low in untreated eyes and did not increase following laser treatment. Conclusion. The results show that MMP-2 mRNA expression is increased in experimental CNV, and support of a role for MMP-2 in the development of CNV in age-related macular degeneration.