Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization
Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization
复制标题
DOI：
10.1111/pim.12091
复制
发表时间：
2014-02-01
影响因子：
2.2
通讯作者：
Fonseca, C. T.
中科院分区：
文献类型：
--
作者：
de Melo, T. T.;de Sena, I. C.;Fonseca, C. T.
曼氏血吸虫童虫外皮（Smteg）在触发宿主免疫反应中发挥重要作用，用弗氏佐剂配制的Smteg或明矾+CpG免疫小鼠可诱导针对曼氏血吸虫感染的部分保护作用，并增加抗体产生。在这项研究中，我们研究了这些抗体在体外和体内杀死寄生虫的作用。我们证明，这些抗体能够结合到曼氏血吸虫最近转化的童虫表面，并且这些抗体显著增加体外补体激活杀死的童虫百分比。被动转移的免疫血清减少寄生虫的负担和数量的鸡蛋被困在小鼠的器官，收到含有抗Smteg抗体的血清。这些结果表明，特异于表面被膜抗原的抗体参与用Smteg免疫的小鼠中的寄生虫消除。
The Schistosoma mansoni schistosomula tegument (Smteg) plays an important role in triggering the host immune response and mice immunization with Smteg formulated with Freund's adjuvant or alum+CpG induce partial protection against S.mansoni infection associated with an increased antibody production. In this study, we investigated the role of these antibodies in parasite killing both in vitro and in vivo. We demonstrated that these antibodies were able to bind to the surface of S.mansoni recently transformed schistosomula and that these antibodies significantly increase the percentage of schistosomula killed in vitro by complement activation. Passive transference of immune sera decreased the parasite burden and the number of eggs trapped in the organs of mice that received sera containing anti-Smteg antibodies. These results demonstrate that antibodies specific to surface tegumental antigens are involved in parasite elimination in mice immunized with Smteg.