Podophyllotoxin and Deoxypodophyllotoxin in Juniperus bermudiana and 12 Other Juniperus Species: Optimization of Extraction, Method Validation, and Quantification
Podophyllotoxin and Deoxypodophyllotoxin in Juniperus bermudiana and 12 Other Juniperus Species: Optimization of Extraction, Method Validation, and Quantification
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DOI：
10.1021/jf201410p
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发表时间：
2011-08-10
影响因子：
6.1
通讯作者：
Hano, Christophe
中科院分区：
文献类型：
--
作者：
Renouard, Sullivan;Lopez, Tatiana;Hano, Christophe
木脂素类鬼臼毒素和脱氧鬼臼毒素是在癌症治疗中具有有效药物应用的次级代谢物。然而，从其目前的天然来源，桃儿七，供应鬼臼毒素变得越来越成问题，因此迫切需要替代来源。到目前为止，在一些杜松属物种中发现了鬼臼毒素和脱氧鬼臼毒素，尽管在大多数情况下含量很低。此外，提取方案值得优化。本研究旨在开发和验证一种有效的提取方案，鬼白毒素和脱氧鬼白毒素从刺柏属物种，并将其应用于13刺柏属物种，其中一些以前从未分析过。刺柏用于开发和验证鬼臼毒素和脱氧鬼臼毒素的提取方案，允许提取率高达22.6 mg/g DW的鬼臼毒素和4.4 mg/g DW的脱氧鬼臼毒素，这是在刺柏叶提取物中发现的最高值。建立并验证了优化的提取方案和DAD或MS检测的HPLC分离，以研究其他12种杜松地上部分中的鬼臼毒素和脱氧鬼臼毒素含量。这使得在报告含有这两种化合物的一些物种中获得更高的产量，或者首次报告在其他一些物种中出现这些化合物。这种有效的协议允许有效提取鬼臼毒素和脱氧鬼臼毒素从刺柏属物种的地上部分，因此可能构成这些有价值的代谢产物的有趣的替代来源。
The lignans podophyllotoxin and deoxypodophyllotoxin are secondary metabolites with potent pharmaceutical applications in cancer therapy. However, the supply of podophyllotoxin from its current natural source, Podophyllum hexandrum, is becoming increasingly problematic, and alternative sources are therefore urgently needed. So far, podophyllotoxin and deoxypodophyllotoxin have been found in some Juniperus species, although at low levels in most cases. Moreover, extraction protocols deserve optimization. This study aimed at developing and validating an efficient extraction protocol of podophyllotoxin and deoxypodophyllotoxin from Juniperus species and applying it to 13 Juniperus species, among which some had never been previously analyzed. Juniperus bermudiana was used for the development and validation of an extraction protocol for podophyllotoxin and deoxypodophyllotoxin allowing extraction yields of up to 22.6 mg/g DW of podophyllotoxin and 4.4 mg/g DW deoxypodophyllotoxin, the highest values found in leaf extract of Juniperus. The optimized extraction protocol and HPLC separation from DAD or MS detections were established and validated to investigate podophyllotoxin and deoxypodophyllotoxin contents in aerial parts of 12 other Juniperus species. This allowed either higher yields to be obtained in some species reported to contain these two compounds or the occurrence of these compounds in some other species to be reported for the first time. This efficient protocol allows effective extraction of podophyllotoxin and deoxypodophyllotoxin from aerial parts of Juniperus species, which could therefore constitute interesting alternative sources of these valuable metabolites.