Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine
Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine
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DOI：
10.1109/10.827289
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发表时间：
2000-03-01
影响因子：
4.6
通讯作者：
Leckband, DE
中科院分区：
文献类型：
--
作者：
Branch, DW;Wheeler, BC;Leckband, DE
为了使神经元在培养中附着并保持精确的微模式数周，需要确定在延长培养时间内保持不允许的背景分子。对聚乙二醇（PEG）或氨基酸丝氨酸（C3H7NO3）的非允许背景分子进行了评价。前景区域用3、5或10 μ m的聚d -赖氨酸（PDL）细胞系进行微印，PDL促进神经附着和生长，培养29天后，前景的适应性，或所有活体细胞停留在所需PDL表面的比例，丝氨酸平均为86%，PEG为90%，只有小幅下降。背景顺应性，即图案背景中没有神经突延伸的正方形面积的比例，分别从40%和55%（中等培养）的高点下降到5.5%和12%（29天）。培养物的图像表明，PEG作为非允许底物明显更有效。我们的结论是，这些材料，特别是PES，足以维持神经元的长期模式培养。我们认为这是第一个高质量的培养神经元长期模式的报告。
For neurons to attach and remain in precise micropatterns for weeks in culture, background molecules that remain nonpermissive for extended culture durations need to be identified. Nonpermissive background molecules of either polyethylene glycol (PEG) or the amino acid serine (C3H7NO3) were evaluated. The foreground regions were microstamped with 3-, 5-, or 10-mu m lines of poly-D-lysine (PDL), which promotes neural attachment and growth, After 29 days in culture the foreground compliance, or the fraction of all live somata which rested on the desired PDL surface, averaged 86% for serine and 90% for PEG with only a small decline. The background compliance, or the fraction of square areas in the pattern background which were free of neurite extension, declined from highs of 40% and 55% (midculture) to 5.5% and 12% (29 days) for serine and PEG, respectively, Images of the cultures suggest that PEG is significantly more effective as a nonpermissive substrate. We conclude that these materials, especially PES, are adequate for the maintenance of long-term patterned cultures of neurons. We believe that this is the first report of high-quality long-term patterning of cultured neurons.