Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain
Morphometric analysis of the AMPA-type neurons in the Deiters vestibular complex of the chick brain
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DOI：
10.1016/j.jchemneu.2008.02.003
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Toledo, Claudio A. B.
中科院分区：
文献类型：
--
作者：
Passetto, Maria F.;Britto, Luiz R. G.;Toledo, Claudio A. B.
以鸡（Gallus Gallus）为研究对象，研究了哺乳动物前庭外侧核（LVN）的鸟类对应体Deiter’s vestibular complex （CD）的背核（dND）和腹核（vND）的后脑神经元ampa型谷氨酸受体（GluR）亚基的类型和免疫标记模式。结果显示，两组细胞的神经元GluR1表达均较差。vND、GluR2/3+和GluR4+标记没有显示区域或神经元大小的偏好，尽管大多数神经元在75%左右。表达GluR2/3的dND神经元主要在85%左右，中型到大型的占85%，主要分布在吻侧极内侧和尾侧极外侧。大多数含有GluR4的dND神经元也在75%左右，更大（70%是大而巨型），其分布似乎与GluR2/3+神经元的分布互补。这种不同的排列表明DC核内和DC核之间的功能差异，也表明这种差异可能归因于GluRs亚基组成的多样性。讨论讨论了鸟类直流管与哺乳动物LVN在形态学和功能上的相关性。为了将这些数据纳入现代比较方法，我们建议对鸟类的dND和vND划分采用类似的命名法，即dLVN和vLVN。(C) 2008 Elsevier B.V.版权所有
Chicken (Gallus gallus) brains were used to investigate the typology and the immunolabel pattern for the subunits composing the AMPA-type glutamate receptors (GluR) of hindbrain neurons of the dorsal (dND) and ventral nuclei (vND) of the Deiter's vestibular complex (CD), which is the avian correspondent of the lateral vestibular nucleus (LVN) of mammals. Our results revealed that neurons of both divisions were poor in GluR1. The vND, the GluR2/3+ and GluR4+ label presented no area or neuronal size preference, although most neurons were around 75%. The dND neurons expressing GluR2/3 are primarily around 85%, medium to large-sized 85%, and predominantly 60% located in the medial portion of the rostral pole and in the lateral portion of the caudal pole. The majority of dND neurons containing GluR4 are also around 75%, larger (70% are large and giant), exhibiting a distribution that seems to be complementary to that of GluR2/3+ neurons. This distinct arrangement indicates functional differences into and between the DC nuclei, also signaling that such variation could be attributed to the diverse nature of the subunit composition of the GluRs. Discussion addresses the morphological and functional correlation of the avian DC with the LVN of mammals in addition to the high morphological correspondence, To include these data into the modern comparative approach we propose to adopt a similar nomenclature for the avian divisions dND and vND that could be referred as dLVN and vLVN. (C) 2008 Elsevier B.V. All rights reserved.