Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation.
Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation.
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钙调蛋白结合蛋白 CBP60g 在拟南芥花青素积累中发挥负调节作用
DOI：
10.1371/journal.pone.0173129
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Wang R
中科院分区：
文献类型：
--
作者：
Zou B;Wan D;Li R;Han X;Li G;Wang R
花青素是一种黄酮类化合物，通常积累在花和果实中，使它们色彩鲜艳。花色素苷的积累是通过花色素苷调控基因和生物合成基因的时空表达调控的。CBP60g是一种钙调素结合蛋白，以前已被证明在病原体抗性、耐旱性和阿坝敏感性中具有作用。在本研究中，我们发现CBP 60g抑制由干旱、蔗糖和激动素诱导的花色素苷积累。CBP60g基因的表达模式与花色素苷积累组织一致。实时荧光定量PCR分析表明，CBP60 g下调了花青素生物合成基因CHS、CHI和DFR，以及MBW复合体的两个成员，MYB转录因子PAP1和bHLH转录因子TT8。
Anthocyanins, a kind of flavonoid, normally accumulate in the flowers and fruits and make them colorful. Anthocyanin accumulation is regulated via the different temporal and spatial expression of anthocyanin regulatory and biosynthetic genes. CBP60g, a calmodulin binding protein, has previously been shown to have a role in pathogen resistance, drought tolerance and ABA sensitivity. In this study, we found that CBP60g repressed anthocyanin accumulation induced by drought, sucrose and kinetin. The expression pattern of CBP60g was in accordance with the anthocyanin accumulation tissues. Real-time qPCR analysis revealed that the anthocyanin biosynthetic genes CHS, CHI and DFR, as well as two members of MBW complex, PAP1, a MYB transcription factor, and TT8, a bHLH transcription factor, were down regulated by CBP60g.