LAMININ IS ASSOCIATED WITH THE NEURITE OUTGROWTH-PROMOTING FACTORS FOUND IN CONDITIONED MEDIA
LAMININ IS ASSOCIATED WITH THE NEURITE OUTGROWTH-PROMOTING FACTORS FOUND IN CONDITIONED MEDIA
复制标题
DOI：
10.1073/pnas.82.7.2183
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
REICHARDT, LF
中科院分区：
文献类型：
--
作者：
LANDER, AD;FUJII, DK;REICHARDT, LF
来自许多细胞类型 (BCE) 的条件培养基 (CM) 含有一种因子，可以吸附到聚阳离子基质上，并导致铺在该基质上的神经元快速而大量地延伸神经突。细胞外基质糖蛋白层粘连蛋白与基质结合时，会引起神经元的类似反应。在本报告中，研究了 6 个含有神经突生长促进因子的 CM。使用亲和纯化的抗层粘连蛋白抗体进行的免疫沉淀表明，层粘连蛋白存在于所有CM中，并且当它被选择性去除时，每个CM中的神经突生长促进活性相应丧失。纯化的层粘连蛋白的抗体未能阻断 5 个测试的 CM 的生长促进活性，即使这些抗体在同一测定中阻断了纯化的层粘连蛋白的生长促进活性。该结果可以反映 CM 衍生的层粘连蛋白和用于生成抗体的纯化层粘连蛋白之间的氨基酸序列或蛋白质修饰的差异。或者，它可能反映了以下事实：其他分子与 CM 中的层粘连蛋白结合，并可能干扰抗体与层粘连蛋白上对生物活性重要的位点的结合。
Conditioned media (CM) from many cell types (BCE) contain a factor that can adsorb to a polycationic substratum and cause neurons plated on that substratum to extend neurites rapidly and profusely. The extracellular matrix glycoprotein laminin, when bound to a substratum, elicits a similar response by neurons. In this report, 6 CM that contain a neurite outgrowth-promoting factor were studied. Immunoprecipitation with affinity-purified anti-laminin antibodies demonstrated that laminin is present in all of them, and when it was selectively removed, there was a corresponding loss of neurite outgrowth-promoting activity in each CM. Antibodies to purified laminin failed, to block the outgrowth-promoting activity of 5 of the CM tested, even though these antibodies blocked the outgrowth promoting activity of purified laminin in the same assay. This result could reflect differences in amino acid sequence or protein modification between CM-derived laminin and the purified laminin used in generating antibodies. Alternatively, it could reflect the fact that other molecules bind to laminin in CM and could interfere with the binding of antibodies to sites on laminin that are important for biological activity.