Abattoir-based estimates of mycobacterial infections in Cameroon.
Abattoir-based estimates of mycobacterial infections in Cameroon.
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DOI：
10.1038/srep24320
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发表时间：
2016-04-14
影响因子：
4.6
通讯作者：
Bronsvoort BM
中科院分区：
文献类型：
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作者：
Egbe NF;Muwonge A;Ndip L;Kelly RF;Sander M;Tanya V;Ngwa VN;Handel IG;Novak A;Ngandalo R;Mazeri S;Morgan KL;Asuquo A;Bronsvoort BM
分枝杆菌引起的主要疾病包括人类结核病、牛结核病和约翰氏病。在牲畜中，优势种是引起牛结核杆菌（bTB）的牛分枝杆菌，这是一种具有全球人畜共患重要性的疾病。在这项研究中，我们估计了喀麦隆屠宰牛中分枝杆菌的流行率。在喀麦隆的四个屠宰场进行了一项横断面研究，共检查了2,346头牛。每只动物最多收集三个病变以供进一步研究，并从随机取样的未病变动物中收集咽后淋巴结。样品在Lowenstein Jensen培养基和BACTEC MGIT 960系统上培养，并使用Hain®基因型试剂盒进行鉴定。在巴门达、恩oundere、Garoua和Maroua屠宰场，共有206 /2,346头牛被鉴定出btb样病变，分别占4.0%（45/1,129）、11.3%（106/935）、23.8%（38/160）和14.8%（18/122）。4个屠宰场牛支原体最小流行率分别为2.8%（1.9 ~ 3.9）、7.7%（6.1 ~ 9.6）、21.3%（15.2 ~ 28.4）和13.1%（7.7 ~ 20.4）。在未损伤的动物身上发现1株结核分枝杆菌和3株牛分枝杆菌。牛支原体的高流行率引起了公共卫生关注，并限制了在这种情况下的潜在控制选择，因为没有可行的疫苗作为替代。
Mycobacteria cause major diseases including human tuberculosis, bovine tuberculosis and Johne’s disease. In livestock, the dominant species is M. bovis causing bovine tuberculosis (bTB), a disease of global zoonotic importance. In this study, we estimated the prevalence of Mycobacteria in slaughter cattle in Cameroon. A total of 2,346 cattle were examined in a cross-sectional study at four abattoirs in Cameroon. Up to three lesions per animal were collected for further study and a retropharyngeal lymph node was collected from a random sample of non-lesioned animals. Samples were cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and identified using the Hain® Genotype kits. A total of 207/2,346 cattle were identified with bTB-like lesions, representing 4.0% (45/1,129), 11.3% (106/935), 23.8% (38/160) and 14.8% (18/122) of the cattle in the Bamenda, Ngaoundere, Garoua and Maroua abattoirs respectively. The minimum estimated prevalence of M. bovis was 2.8% (1.9–3.9), 7.7% (6.1–9.6), 21.3% (15.2–28.4) and 13.1% (7.7–20.4) in the four abattoirs respectively. One M. tuberculosis and three M. bovis strains were recovered from non-lesioned animals. The high prevalence of M. bovis is of public health concern and limits the potential control options in this setting without a viable vaccine as an alternative.