CONTINUOUS SPECTROPHOTOMETRIC ASSAY FOR ANGIOTENSIN CONVERTING ENZYME
CONTINUOUS SPECTROPHOTOMETRIC ASSAY FOR ANGIOTENSIN CONVERTING ENZYME
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DOI：
10.1016/0003-2697(79)90769-3
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
RIORDAN, JF
中科院分区：
文献类型：
--
作者：
HOLMQUIST, B;BUNNING, P;RIORDAN, JF
采用符合血管紧张素转换酶（二肽基羧肽酶，EC 3.4.15.1）已知底物特异性的呋喃丙烯酰三肽[来自兔肺]，在可见区对该肽酶进行连续分光光度测定。该分析是基于在底物水解产生呋喃丙烯酰阻断的氨基酸和二肽时发生的吸收光谱的蓝移。在测试的各种呋喃丙烯酰三肽中，呋喃丙烯酰- l-苯丙酰酰甘氨酸表现出最适合血管紧张素转换酶常规测定的特征。
Furanacryloyl tripeptides conforming to the known substrate specificity of the angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1) [from rabbit lung] were employed to provide a continuous spectrophotometric assay for this peptidase in the visible region. The assay is based on a blue shift of the absorption spectrum that occurs upon hydrolysis of the substrate to produce a furanacryloyl-blocked amino acid and a dipeptide. Of the various furanacryloyl tripeptides tested, furanacryloyl-L-phenylalanylglycylglycine exhibits the most suitable characteristics for routine assays of angiotensin converting enzyme.