HEPATITIS-C VIRUS-RNA AND HEPATITIS-B VIRUS-DNA IN SERUM AND LIVER OF PATIENTS WITH FULMINANT-HEPATITIS
HEPATITIS-C VIRUS-RNA AND HEPATITIS-B VIRUS-DNA IN SERUM AND LIVER OF PATIENTS WITH FULMINANT-HEPATITIS
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DOI：
10.1016/0016-5085(93)90425-c
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发表时间：
1993-02-01
期刊：
影响因子：
29.4
通讯作者：
BRECHOT, C
中科院分区：
文献类型：
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作者：
FERAY, C;GIGOU, M;BRECHOT, C
背景：丙型肝炎病毒（HCV）在重型肝炎发生发展中的作用尚不清楚，方法：采用聚合酶链反应（PCR）技术检测40例重型肝炎和亚重型肝炎患者血清和肝脏中的HCV RNA和HBV DNA，结果：23例不明原因的B肝炎表面抗原（HBsAg）阴性肝炎患者均未检出HCV RNA，其中10例为HCV RNA阳性，10例为HBV DNA阳性。在这23例受试者中的1例中发现HBV DNA。17例HBeAg阳性肝炎患者中8例HCV RNA阳性。在后者中，5例有抗B核心抗原（抗-HBc）的免疫球蛋白n（IG）M抗体，因此是HBV和HCV的急性合并感染，而另外3例无抗-HBc IgM抗体的患者是HCV的重叠感染。HCV RNA和负链HCVRNA.Conclusion的高滴度的检测表明，在肝脏中的HCV的活跃复制：因此，这项调查显示没有证据表明丙型肝炎病毒感染的暴发性肝炎的散发病例中的作用，没有明确的病因。然而，这表明HCV可能与相当数量的HBV相关的暴发性肝炎患者有关。
Background:The role of hepatitis C virus (HCV) in the development of fulminant hepatitis is poorly understood.Methods:The polymerase chain reaction was used to detect HCV RNA and hepatitis B virus (HBV) DNA in serum and liver of 40 patients with fulminant or subfulminant hepatitis.Results:HCV RNA was detected in none of the 23 subjects with hepatitis B surface antigen (HBsAg)-negative hepatitis of unknown etiology. HBV DNA was found in 1 of these 23 subjects. In contrast, 8 of the 17 patients with HBsAg-positive hepatitis were HCV RNA positive. Among the latter, 5 had immunoglobulfn (Ig) M antibody to hepatitis B core antigen (anti-HBc) and thus were acutely coinfected by HBV and HCV, whereas 3 others without IgM anti-HBc had superinfection by HCV. The active replication of HCV in the liver was shown by detection of high titers of HCV RNA and of negative-stranded HCV RNA.Conclusion:This investigation therefore shows no evidence of a role for HCV infection in sporadic cases of fulminant hepatitis without defined etiology. However, it suggests that HCV might be implicated in a significant number of patients with HBV-related fulminant hepatitis.