Differential expression of the mitochondrial gene cytochrome oxidase ii in benign and malignant breast tissue
Differential expression of the mitochondrial gene cytochrome oxidase ii in benign and malignant breast tissue
复制标题
良恶性乳腺组织中线粒体基因细胞色素氧化酶ii的差异表达
DOI：
10.1002/path.1711680203
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. Varley
中科院分区：
文献类型：
--
作者：
M. Sharp;S. Adams;R. Walker;W. J. Brammar;J. Varley
对乳腺良性肿瘤和恶性肿瘤的基因表达水平进行了比较分析。cDNA文库的差异筛选发现，线粒体基因组的四个基因在两种组织中表达水平不同，但进一步的研究表明，只有编码细胞色素c氧化酶（COII）亚基2的基因在癌中的表达水平明显高于纤维腺瘤。编码NADH脱氢酶亚基2和4以及F0F1 atp酶亚基6的线粒体基因在癌和纤维腺瘤中未发现差异表达。原位杂交结果显示，这四个基因均在人乳腺癌上皮细胞中表达。COII基因表达水平也与肿瘤分级相关。这些肿瘤的线粒体DNA没有明显的变化。这些结果对纤维腺瘤和癌之间行为差异的可能含义进行了讨论。
Comparative analysis of expression levels of genes in benign and malignant tumours of the breast has been performed. Differential screening of cDNA libraries identified four genes of the mitochondrial genome as being expressed at different levels in the two tissues compared, but further investigations showed that only the gene encoding subunit 2 of cytochrome c oxidase (COII) is expressed at significantly higher levels in carcinomas compared with fibroadenomas. The mitochondrial genes encoding subunits 2 and 4 of NADH dehydrogenase, and subunit 6 of F0F1 ATPase, were not found to be differentially expressed in carcinomas and fibroadenomas. All four genes were expressed in the epithelium of human breast carcinomas, as shown by in situ hybridization. The expression level of the COII gene is also correlated with carcinoma grade. No gross alterations to the mitochondrial DNA from these tumours could be detected. The possible implications of these results on the behavioural differences between fibroadenomas and carcinomas are discussed.
线粒体细胞色素c氧化酶在人结肠细胞分化、转化和结肠癌风险中的表达。
影响因子：
11.2
作者：
Heerdt,BG;Halsey,HK;Lipkin,M;Augenlicht,LH
通讯作者：
Augenlicht,LH