DIRECT INHIBITION OF THE YEAST CYCLIN-DEPENDENT KINASE CDC28-CLN BY FAR1
DIRECT INHIBITION OF THE YEAST CYCLIN-DEPENDENT KINASE CDC28-CLN BY FAR1
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DOI：
10.1126/science.8066461
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发表时间：
1994-08-26
期刊：
影响因子：
56.9
通讯作者：
HERSKOWITZ, I
中科院分区：
文献类型：
--
作者：
PETER, M;HERSKOWITZ, I
抗丝裂原α-因子将酿酒酵母细胞周期阻滞在G（1），这是通过激活导致细胞周期蛋白依赖性激酶Cdc 28-Cln抑制的信号转导途径介导的。Farl蛋白是细胞周期阻滞所需的，并与Cdc 28-Cln复合物相关。Cdc 28-Cln的激酶活性在体内和体外均被Far 1直接抑制，从而证明Far 1通过抑制G（1）细胞周期蛋白依赖性激酶在α-因子反应途径的最后一步起作用。
Cell cycle arrest of Saccharomyces cerevisiae in G(1) by the antimitogen alpha-factor is mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Farl protein is required for cell cycle arrest and associates with the Cdc28-Cln complex. The kinase activity of Cdc28-Cln was directly inhibited by Far1 both in vivo and in vitro, thus demonstrating that Far1 acts at the final step in the alpha-factor response pathway by inhibiting a G(1) cyclin-dependent kinase.