INTRACEREBROVENTRICULARLY APPLIED PEPTIDASE INHIBITORS INCREASE ENDOGENOUS ANGIOTENSIN LEVELS
INTRACEREBROVENTRICULARLY APPLIED PEPTIDASE INHIBITORS INCREASE ENDOGENOUS ANGIOTENSIN LEVELS
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DOI：
10.1016/0006-8993(90)90819-w
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发表时间：
1990-10-08
期刊：
影响因子：
2.9
通讯作者：
WRIGHT, JW
中科院分区：
文献类型：
--
作者：
BATT, CM;JENSEN, LL;WRIGHT, JW
大鼠通过脑室内输注各种组合的氨肽酶抑制剂amastatin（AM）和bestatin（BE）以及羧肽酶抑制剂Plummer's（PL），即PL单独、AM+BE和AM+BE+PL组成的混合物。记录血压反应，并对输注后脑脊液（CSF）样本进行内源性血管紧张素水平的放射免疫测定。结果表明CSF血管紧张素增加约1.5倍。与对照水平相比，当PL被输注时;伴随AM+BE给药的增加;当所有3种抑制剂作为鸡尾酒输注时测量升高。伴随的血压升高伴随血管紧张素浓度增加。我们的结论是，内源性血管紧张素水平可以显着增加，在心室空间时，这些抑制剂的组合被用来保护的氨基和羧基末端的血管紧张素分子酶降解。
Rats received the aminopeptidase inhibitors amastatin (AM) and bestatin (BE), and carboxypeptidase inhibitor Plummer''s (PL) via intracerebroventricular infusion in various combinations, i.e. PL alone, AM+BE, and a cocktail consisting of AM+BE+PL. Blood pressure responses were recorded and a postinfusion sample of cerebrospinal fluid (CSF) was radioimmunoassayed for endogenous angiotensin levels. Results indicate that CSF angiotensin was increased approximately 1.5 .times. over control levels when PL was infused; a 2.5 .times. increase accompanied AM+BE administration; and a 10.3 .times. elevation was measured when all 3 inhibitors wee infused as a cocktail. Concomitant elevations in blood pressure accompanied increased concentations of angiotensin. We conclude that endogenous levels of angiotensin can be significantly increased in the ventricular space when a combination of these inhibitors is utilized to protect both the amino and carboxyl terminals of the angiotensin molecule from enzymatic degradation.