Rapid seven-hour fecal coliform test
Rapid seven-hour fecal coliform test
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七小时粪便大肠菌群快速检测
DOI：
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发表时间：
1979
影响因子：
4.4
通讯作者：
E. Geldreich
中科院分区：
文献类型：
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作者：
D. Reasoner;J. Blannon;E. Geldreich
建立了一种快速检测水中粪大肠菌群（FC）的7小时快速试验方法。该膜过滤器试验采用轻度缓冲乳糖基培养基（m-7-h FC培养基）与灵敏的pH指示剂系统组合。在41.5 ℃下孵育7至7.25 h后，FC菌落在浅紫色背景下呈现黄色。FC测试结果的比较表明，平均验证FC计数比（7小时FC计数/24小时FC计数）为1.08的地表水样品。平均FC计数比（7小时FC计数/24小时FC计数）为unchlorinater废水范围从1.95到5.05。来自m-7-h FC培养基的黄色FC菌落的验证平均为97%。密歇根湖游泳海滩水样的现场测试数据显示，未经验证的7小时FC计数平均为96%的24小时FC计数。7小时FC试验被认为是适合于检查地表沃茨和未氯化的污水，并可以作为一个紧急测试检测污水或粪便污染的饮用水。
A rapid 7-h fecal coliform (FC) test for the detection of FC in water has been developed. This membrane filter test utilizes a lightly buffered lactose-based medium (m-7-h FC medium) combined with a sensitive pH indicator system. FC colonies appeared yellow against a light purple background after incubation at 41.5 degrees C for 7 to 7.25 h. Comparison of FC test results showed that the mean verified FC count ratio (7-h FC count/24-h FC count) for surface water samples was 1.08. The mean FC count ratio (7-h FC count/24-h FC count) for unchlorinater wastewater ranged from 1.95 to 5.05. Verification of yellow FC colonies from m-7-h FC medium averaged 97%. Data from field tests on Lake Michigan bathing beach water samples showed that unverified 7-h FC counts averaged 96% of the 24-h FC counts. The 7-h FC test was found to be suitable for the examination of surface waters and unchlorinated sewage and could serve as an emergency test for detection of sewage or fecal contamination of potable water.