Mechanisms governing the activation and trafficking of yeast G protein-coupled receptors.
Mechanisms governing the activation and trafficking of yeast G protein-coupled receptors.
复制标题
控制酵母 G 蛋白偶联受体激活和运输的机制。
DOI：
10.1091/mbc.9.4.885
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发表时间：
1998
影响因子：
3.3
通讯作者：
Blumer,KJ
中科院分区：
文献类型：
--
作者：
Stefan,CJ;Overton,MC;Blumer,KJ
We have addressed the mechanisms governing the activation and trafficking of G protein-coupled receptors (GPCRs) by analyzing constitutively active mating pheromone receptors (Ste2p and Ste3p) of the yeastSaccharomyces cerevisiae. Substitution of the highly conserved proline residue in transmembrane segment VI of these receptors causes constitutive signaling. This proline residue may facilitate folding of GPCRs into native, inactive conformations, and/or mediate agonist-induced structural changes leading to G protein activation. Constitutive signaling by mutant receptors is suppressed upon coexpression with wild-type, but not G protein coupling-defective, receptors. Wild-type receptors may therefore sequester a limiting pool of G proteins; this apparent “precoupling” of receptors and G proteins could facilitate signal production at sites where cell surface projections form during mating partner discrimination. Finally, rather than being expressed mainly at the cell surface, constitutively active pheromone receptors accumulate in post-endoplasmic reticulum compartments. This is in contrast to other defective membrane proteins, which apparently are targeted by default to the vacuole. We suggest that the quality-control mechanism that retains receptors in post-endoplasmic reticulum compartments may normally allow wild-type receptors to fold into their native, fully inactive conformations before reaching the cell surface. This may ensure that receptors do not trigger a response in the absence of agonist.
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DOI：
10.1016/s0021-9258(18)53442-6
发表时间：
1993-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Samama;S. Cotecchia;T. Costa;R. Lefkowitz
通讯作者：
P. Samama;S. Cotecchia;T. Costa;R. Lefkowitz
影响因子：
2.9
作者：
KAUSHAL, S;KHORANA, HG
通讯作者：
KHORANA, HG
激动剂和拮抗剂与α2肾上腺素能受体结合的机制：预偶联受体-鸟嘌呤核苷酸蛋白复合物的证据。
影响因子：
2.9
作者：
R. Neubig;R. Gantzos;William J. Thomsen
通讯作者：
William J. Thomsen
影响因子：
--
作者：
R. Sikorski;J. Boeke
通讯作者：
R. Sikorski;J. Boeke
转染的 PC-12 细胞膜中 α-2B 肾上腺素受体和 G 蛋白的预偶联：百日咳毒素和赖氨酸定向交联剂的影响。
DOI：
--
发表时间：
1994
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Shi,AG;Deth,RC
通讯作者：
Deth,RC