A guinea pig model for Lyme disease.
A guinea pig model for Lyme disease.
复制标题
莱姆病豚鼠模型。
DOI：
10.1128/iai.61.11.4777-4784.1993
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Goodman,JL
中科院分区：
文献类型：
--
作者：
Sonnesyn,SW;Manivel,JC;Johnson,RC;Goodman,JL
我们报告说，远交哈特利豚鼠对伯氏疏螺旋体易感。我们从≤ 3月龄时接种的60只豚鼠中的57只（95%）中回收了螺旋体。相反，当>或= 6月龄时接种的动物对感染具有抗性，其定义为接种后>或= 4周时微生物的恢复。感染广泛传播：B。在接种后4周内，从83%的膀胱、64%的膝关节、57%的心脏、48%的脾和38%的脊髓中检测到伯氏菌。组织病理学变化常见于心脏（88%）（优先累及纤维环附近的神经周组织）和膀胱（76%），少数脊髓（13%）和膝关节（9%）也出现组织病理学变化。Western免疫印迹显示免疫球蛋白G对B的反应。本发明的方法可用于检测伯氏螺旋体，特别是24-、31-（OspA）、39-和41-kDa（鞭毛蛋白）抗原。随着时间的推移，大多数组织中的感染被清除;在接种后≤ 4周，63%的豚鼠组织中回收了螺旋体，而在接种后≥ 8周，仅32%的豚鼠组织中回收了螺旋体（P < 0.001）。一个例外是未能从感染的膝关节清除螺旋体，其中90%的培养阳性，即使在接种后≥ 8周进行评估。豚鼠为研究莱姆病中宿主-螺旋体相互作用提供了一种新的模型。
We report that outbred Hartley guinea pigs are susceptible to Borrelia burgdorferi. We recovered spirochetes from 57 of 60 (95%) guinea pigs inoculated when < or = 3 months of age. In contrast, animals inoculated when > or = 6 months of age were resistant to infection as defined by recovery of organisms at > or = 4 weeks postinoculation. Infection was widely disseminated: B. burgdorferi was recovered from 83% of bladders, 64% of knee joints, 57% of hearts, 48% of spleens, and 38% of spinal cords examined within 4 weeks of inoculation. Histopathologic changes were common in the heart (88%) (preferential involvement of perineural tissues near the annulus fibrosus) and bladder (76%) and were also noted in a minority of spinal cords (13%) and knee joints (9%). Western immunoblots demonstrated an immunoglobulin G response to B. burgdorferi, particularly to the 24-, 31- (OspA), 39-, and 41-kDa (flagellin) antigens. Infection was cleared from most tissues with the passage of time; spirochetes were recovered from 63% of tissues removed from guinea pigs at < or = 4 weeks after inoculation but from only 32% at > or = 8 weeks postinoculation (P < 0.001). An exception was the failure to clear spirochetes from infected knees, 90% of which were culture positive even when evaluated at > or = 8 weeks postinoculation. The guinea pig provides a new model useful for studying host-spirochete interactions in Lyme disease.