Delivery of Differentiation Factors by Mesoporous Silica Particles Assists Advanced Differentiation of Transplanted Murine Embryonic Stem Cells
Delivery of Differentiation Factors by Mesoporous Silica Particles Assists Advanced Differentiation of Transplanted Murine Embryonic Stem Cells
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DOI：
10.5966/sctm.2013-0072
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发表时间：
2013-11-01
影响因子：
6
通讯作者：
Kozlova, Elena N.
中科院分区：
文献类型：
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作者：
Garcia-Bennett, Alfonso E.;Kozhevnikova, Mariya;Kozlova, Elena N.
干细胞移植为神经系统受损细胞的替换带来了很大的希望。然而，移植后的长期存活率差以及干细胞在体内分化为特化细胞类型的能力不足仍然是临床应用的主要障碍。在这里，我们报告了一种新的技术方法的发展，用于使用专门设计的二氧化硅纳米多孔颗粒将外源性营养因子模拟物局部递送到移植细胞。我们证明，分别将Cintrofin和Gliafin（已建立的纤毛神经营养因子和胶质细胞系衍生的神经营养因子的肽模拟物）与这些颗粒一起递送，不仅能够从移植的胚胎干细胞中实现运动神经元的强大功能分化，而且还能使它们在体内长期存活。我们提出，通过介孔纳米颗粒递送生长因子是一种潜在的通用和广泛适用的策略，可用于干细胞衍生物移植后的有效分化和功能整合。
Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.