Expression of multidrug resistance-related transporters in human breast carcinoma.
Expression of multidrug resistance-related transporters in human breast carcinoma.
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DOI：
10.1111/j.1349-7006.2001.tb01115.x
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发表时间：
2001-04
期刊：
影响因子：
--
通讯作者：
Takebayashi Y
中科院分区：
文献类型：
--
作者：
Kanzaki A;Toi M;Nakayama K;Bando H;Mutoh M;Uchida T;Fukumoto M;Takebayashi Y
在43名未经治疗的乳腺癌患者中检测了多药耐药1（MDR 1）基因、多药耐药蛋白1（MRP 1）、肺耐药相关蛋白（LRP）和乳腺癌耐药蛋白（BCRP）的mRNA表达水平，这些基因在体外赋予多药耐药，其中38名患者在手术后接受了基于阿霉素的化疗，以阐明这些基因在体内耐药性中的作用。使用半定量逆转录聚合酶链反应方法在包括至少80%癌细胞的乳腺癌组织中测定mRNA水平。BCRP基因表达水平较低，与MDR 1、MRP 1、LRP基因表达水平无明显差异。MDR 1和MRP 1基因表达相关，BCRP和LRP基因表达与其他基因表达无相关性。这四个基因的表达与年龄、TNM分期、孕激素或雌激素受体状态无关。这四种基因的表达水平与38例接受阿霉素化疗的患者的复发或预后无关。P-糖蛋白（P-gp）/MDR 1、MRP 1和LRP在乳腺癌化疗中的作用可能比BCRP更重要。
The expression levels of mRNA for multidrug resistance 1 (MDR1) gene, multidrug resistance protein 1 (MRP1), lung resistance‐related protein (LRP) and breast cancer resistance protein (BCRP), which confer multidrug resistance in vitro, were examined in 43 untreated breast carcinoma patients, of whom 38 subsequently received doxorubicin‐based chemotherapy after surgery, in order to elucidate the roles of these genes in drug resistance in vivo. The mRNA levels were determined using a semi‐quantitative reverse‐transcription polymerase chain reaction method in breast carcinoma tissues including at least 80% carcinoma cells. The expression level of BCRP gene was low and did not vary markedly in comparison with that of MDR1, MRP1 or LRP gene. The expressions of MDR1 and MRP1 genes were correlated with each other, but the expression of BCRP or LRP gene did not correlate with that of other genes. These four gene expressions were independent of age, TNM categories and the status of progesterone or estrogen receptor. The expression levels of these four genes were not related to the relapse or prognosis of the 38 patients treated with doxorubicin‐based chemotherapy. P‐glycoprotein (P‐gp)/MDRl, MRP1 and LRP may play more important roles than BCRP in chemotherapy of human breast carcinoma.
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多药抗性相关蛋白（MRP）在原发性乳腺癌中表达的预后意义。
影响因子：
8.8
作者：
Nooter, K;Brutel de la Riviere, G;Look, M P;van Wingerden, K E;Henzen-Logmans, S C;Scheper, R J;Flens, M J;Klijn, J G;Stoter, G;Foekens, J A
通讯作者：
Foekens, J A
DOI：
10.1073/pnas.95.26.15665
发表时间：
1998-12-22
影响因子：
11.1
作者：
Doyle, LA;Yang, WD;Ross, DD
通讯作者：
Ross, DD
影响因子：
50.5
作者：
Hegewisch-Becker, S;Staib, F;Hossfeld, DK
通讯作者：
Hossfeld, DK
影响因子：
10.3
作者：
CHARPIN, C;VIELH, P;PIANA, L
通讯作者：
PIANA, L
影响因子：
3.6
作者：
Alvarez, M;Robey, R;Fojo, T
通讯作者：
Fojo, T