Soft x-ray diffraction up to wavelengths of 6.0 Å becomes feasible at ID1 of ESRF
Soft x-ray diffraction up to wavelengths of 6.0 Å becomes feasible at ID1 of ESRF
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DOI：
10.12693/aphyspola.101.603
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发表时间：
2002-05-01
影响因子：
0.7
通讯作者：
Vicat, J
中科院分区：
文献类型：
--
作者：
Carpentier, P;Boesecke, P;Vicat, J
本文描述了最近在ESRF的反常散射光束线ID1上进行的实验，这些实验表明，冷氦气氛为用波长高达6埃的软X射线进行蛋白质结晶学提供了最佳条件。成像板是适合这个波长范围的探测器。与早期的实验相比，改进是相当大的。这一观察结果为利用轻元素如磷、硫、氯和钙在K边的反常弥散开辟了道路，此外，人们还有兴趣利用一些重元素如铀的强烈反常弥散(在其M-V边上有110个反常电子单位)来解决大型生物结构。多波长反常衍射法和反常精细结构衍射法发现了在生物和材料中广泛存在的新的共振标记物。
Recent experiments carried out at the Anomalous Scattering Beam-line ID1 of ESRF are described which show that a cold helium atmosphere provides optimal conditions for protein crystallography with soft X-rays at wavelengths up to 6 Angstrom. Image plates are suitable detectors for this wavelength range. The improvement with respect to earlier experiments is considerable. This observation opens the way to the use of anomalous dispersion at the K-edge of light elements, like phosphorus, sulphur, chlorine and calcium, Moreover, there is an interest to use the strong anomalous dispersion of some heavy elements such as uranium (110 anomalous electron units at its M-V edge) to solve large biological structures. The methods of multiwavelength anomalous diffraction and diffraction anomalous fine structure find new resonant labels which are wide spread in living matter and materials.