Age-related structural abnormalities in the human retina-choroid complex revealed by two-photon excited autofluorescence imaging
Age-related structural abnormalities in the human retina-choroid complex revealed by two-photon excited autofluorescence imaging
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DOI：
10.1117/1.2717522
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Niemz, Markolf H.
中科院分区：
文献类型：
--
作者：
Han, Meng;Giese, Guenter;Niemz, Markolf H.
视网膜色素上皮（RPE）和脉络膜精细地维持光感受器的密集代谢。视网膜色素上皮或脉络膜的功能障碍可能导致视网膜严重损伤。来自人视网膜内源性荧光团的双光子激发的自发荧光（TPEF）提供了一个新的机会来揭示年龄相关性视网膜疾病的明显病理表现之前的视网膜脉络膜复合体中的年龄相关性结构异常。在光感受器层中，黄斑光感受器马赛克的规则性在老化过程中得以保留。在RPE中，增大的脂褐质颗粒显示出显著的蓝移自发荧光，这与黑色素的消耗一致。老年人布鲁赫膜和脉络膜毛细血管中突出的纤维状结构代表脉络膜结构和通透性的改变。TPEF成像既不需要切片也不需要标记，是一种优雅而高效的工具，可以描绘厚、脆弱和不透明的视网膜-脉络膜复合体，并可能为年龄相关性黄斑变性的触发事件提供线索。(C)2007年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
The intensive metabolism of photoreceptors is delicately maintained by the retinal pigment epithelium (RPE) and the choroid. Dysfunction of either the RPE or choroid may lead to severe damage to the retina. Two-photon excited autofluorescence (TPEF) from endogenous fluorophores in the human retina provides a novel opportunity to reveal age-related structural abnormalities in the retinachoroid complex prior to apparent pathological manifestations of age-related retinal diseases. In the photoreceptor layer, the regularity of the macular photoreceptor mosaic is preserved during aging. In the RPE, enlarged lipofuscin granules demonstrate significantly blueshifted autofluorescence, which coincides with the depletion of melanin pigments. Prominent fibrillar structures in elderly Bruch's membrane and choriocapillaries represent choroidal structure and permeability alterations. Requiring neither slicing nor labeling, TPEF imaging is an elegant and highly efficient tool to delineate the thick, fragile, and opaque retina-choroid complex, and may provide clues to the trigger events of age-related macular degeneration. (C) 2007 Society of Photo-Optical Instrumentation Engineers.