Norepinephrine, tri-iodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mRNA during brown adipocyte differentiation
Norepinephrine, tri-iodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mRNA during brown adipocyte differentiation
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DOI：
10.1530/eje.0.1410169
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发表时间：
1999-08-01
影响因子：
5.8
通讯作者：
Santisteban, P
中科院分区：
文献类型：
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作者：
Barroso, I;Benito, B;Santisteban, P
用北方印迹法研究了甘油醛-3-磷酸脱氢酶（GAPDH）基因在棕色脂肪细胞分化过程中的表达。结果表明，在前体细胞向成熟脂肪细胞分化的过程中，GAPDH mRNA表达水平升高，主要发生在分化的初期。胰岛素，三碘甲状腺原氨酸（T-3）和去甲肾上腺素，棕色脂肪组织功能的主要调节剂，上调GAPDH mRNA水平，而视黄酸抑制它们。胰岛素的影响存在于所有的文化天检查，是时间和剂量依赖性，并通过其自身的受体，通过比较胰岛素和胰岛素样生长因子（IGF）-I和-II在这个系统中的效力证明。使用转录抑制剂，放线菌素D，我们证明了T-3，并在较小程度上胰岛素，稳定GAPDH mRNA，放线菌酮的实验表明，这两种激素需要从头蛋白质合成，以实现其效果。使用cAMP类似物，我们表明去甲肾上腺素的作用可能是通过这个第二信使发挥的。在去甲肾上腺素和胰岛素介导的GAPDH mRNA水平诱导之间阐明了GO操作。总之，黑麦已经证明GAPDH mRNA在分化棕色脂肪细胞中受到多因素调节，包括前体细胞的分化和组织的脂肪生成/脂解调节剂。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression was studied in differentiating brown adipocytes, Northern blot analysis showed that GAPDH mRNA levels increased during differentiation of precursor cells into mature adipocytes, mainly in the initial stages of the differentiation process. Insulin, tri-iodothyronine (T-3) and norepinephrine, the main regulators of brown adipose tissue function, upregulated GAPDH mRNA levels, whereas retinoic acid inhibited them. The effect of insulin was present on all culture days examined, was time- and dose-dependent, and was exerted through its own receptors, as demonstrated by comparing insulin and insulin-like growth factor (IGF)-I and -II potencies in this system. Using the transcriptional inhibitor, actinomycin D, we demonstrated that T-3, and to a lesser extent insulin, stabilized GAPDH mRNA, Experiments with cycloheximide indicated that both hormones require de novo protein synthesis to achieve their effects. Using cAMP analogs, we showed that the effect of norepinephrine is probably exerted through this second messenger. Go-operation was elucidated between norepinephrine- and insulin-mediated induction of GAPDH mRNA levels. In summary, rye have demonstrated that GAPDH mRNA is subjected to multifactorial regulation in differentiating brown adipocytes that includes differentiation of precursor cells and the lipogenic/lipolytic regulators of the tissue.