Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ
Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ
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DOI：
10.3389/fnana.2014.00134
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发表时间：
2014-11-21
影响因子：
2.9
通讯作者：
Spehr, Marc
中科院分区：
文献类型：
--
作者：
Ackels, Tobias;von der Weid, Benoit;Spehr, Marc
小鼠犁鼻器（VNO）是一种化学感受结构，可以检测异种和同种的社会线索。基于1型或2型犁鼻受体（V1 Rs/V2 Rs）或甲酰肽受体（EPA）家族成员的大部分单基因表达，犁鼻感觉上皮含有至少三个神经元亚群。虽然V1 R和V2 R表达神经元的各种神经生理学特性已经使用基因工程小鼠模型进行了描述，但最近鉴定的FPR表达犁鼻神经元的基本生物物理学特性尚未研究。在这里，我们采用了一种转基因小鼠品系，共同表达的黄色荧光蛋白与FPR-rs3的增强变体，允许识别和分析FPR-rs3表达神经元在急性VNO组织切片。单神经元电生理记录允许固有的EPA表达的VNO神经元亚群的原型成员的生物物理特性的比较表征。在这项研究中，我们提供了一个深入的分析被动和主动膜的特性，包括几种类型的电压激活的电导和动作电位放电模式的详细表征，在荧光标记与未标记的犁鼻神经元。我们的结果揭示了转基因表达和非表达神经元的基本（电）生理结构的惊人相似性，证实了这种基因工程小鼠模型适用于未来解决犁鼻FPR神经生物学中更专业问题的研究。
The mouse vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Based on largely monogenic expression of either type 1 or 2 vomeronasal receptors (V1Rs/V2Rs) or members of the formyl peptide receptor (EPA) family, the vomeronasal sensory epithelium harbors at least three neuronal subpopulations. While various neurophysiological properties of both V1R- and V2R-expressing neurons have been described using genetically engineered mouse models, the basic biophysical characteristics of the more recently identified FPR-expressing vomeronasal neurons have not been studied. Here, we employ a transgenic mouse strain that coexpresses an enhanced variant of yellow fluorescent protein together with FPR-rs3 allowing to identify and analyze FPR-rs3-expressing neurons in acute VNO tissue slices. Single neuron electrophysiological recordings allow comparative characterization of the biophysical properties inherent to a prototypical member of the EPA-expressing subpopulation of VNO neurons. In this study, we provide an in-depth analysis of both passive and active membrane properties, including detailed characterization of several types of voltage-activated conductances and action potential discharge patterns, in fluorescently labeled vs. unmarked vomeronasal neurons. Our results reveal striking similarities in the basic (electro) physiological architecture of both transgene-expressing and non-expressing neurons, confirming the suitability of this genetically engineered mouse model for future studies addressing more specialized issues in vomeronasal FPR neurobiology.