Mapping of genetic locus for leaf trichome in Brassica oleracea
Mapping of genetic locus for leaf trichome in Brassica oleracea
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甘蓝叶毛状体遗传位点定位
DOI：
10.1007/s00122-017-2936-y
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发表时间：
2017-09-01
影响因子：
5.4
通讯作者：
Qian, Wei
中科院分区：
文献类型：
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作者：
Mei, Jiaqin;Wang, Jinhua;Qian, Wei
通过分子标记定位和SNP微阵列分析，确定了甘蓝叶毛的遗传位点，并开发了一个用于甘蓝叶毛性状育种的功能标记。植物毛状体具有多种功能，特别是在保护植物免受生物和非生物伤害方面。本研究以无毛栽培种B为母本，通过杂交构建了F2分离群体。oleracea（CC，2n = 18）和有毛野生近缘种B。incana（CC，2n = 18）。在1063个F2基因型中检测到无毛和有毛植株之间的1：3分离模式，并且使用149个F2基因型中的267个SSR标记将有毛位点定位在4.3-cM的遗传区域中，对应于染色体C 01上的17.6-Mb基因组区域。为了缩小多毛的遗传区域，将芸苔属60 K SNP珠芯片阵列应用于基因型64个无毛和30个有毛F2植物，导致位于17.6-Mb间隔中的1.04-Mb单峰区域。通过qRT-PCR鉴定了一个候选基因BoTRY，该基因在无毛F2基因型中的表达量显著高于有毛植株。成功地开发了一个切割扩增多态性位点标记来区分有毛和无毛植物之间的BoTRY序列变异。本研究为B猪毛羽的分子育种提供了理论依据。甘蓝。
The genetic locus for leaf trichome was identified via marker-based mapping and SNP microarray assay, and a functional marker was developed to facilitate the breeding for hairiness in Brassica oleracea. Plant trichomes are involved in various functions particularly in protecting plants against some biotic and abiotic damages. In the present study, an F2 segregating population was developed from the cross between a glabrous cultivated B. oleracea (CC, 2n = 18) and a hairy wild relative, B. incana (CC, 2n = 18). A 1:3 segregation pattern between glabrous and hairy plants was detected among 1063 F2 genotypes, and the locus for hairiness was mapped in a 4.3-cM genetic region using 267 SSR markers among 149 F2 genotypes, corresponding to a 17.6-Mb genomic region on chromosome C01. To narrow the genetic region for hairiness, the Brassica 60 K SNP Bead Chip Arrays were applied to genotype 64 glabrous and 30 hairy F2 plants, resulting in a 1.04-Mb single peak region located in the 17.6-Mb interval. A candidate gene, BoTRY, was identified by qRT-PCR which revealed significant higher expression in glabrous F2 genotypes as compared with that in hairy plants. A cleaved amplified polymorphic site marker was successfully developed to distinguish the sequence variations of BoTRY between hairy and glabrous plants. Our study will be helpful for molecular breeding for hairiness in B. oleracea.