Biodegradation of plasticizers by Rhodotorula rubra
Biodegradation of plasticizers by Rhodotorula rubra
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DOI：
10.1002/etc.5620220609
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发表时间：
2003-06
影响因子：
4.1
通讯作者：
J. Gartshore;D. Cooper;J. Nicell
中科院分区：
文献类型：
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作者：
J. Gartshore;D. Cooper;J. Nicell
研究了酵母菌红酵母J‐96‐1 （American Type Culture Collection 9449）在葡萄糖存在下对增塑剂的降解。增塑剂包括常用的己二酸双- 2 -乙基己酯（B(EH)A）、邻苯二甲酸二辛酯（DOP）和对苯二甲酸二辛酯（DOTP），以及不太常用的二苯甲酸二丙二醇酯（D(PG)DB）和二苯甲酸二乙二醇酯（D(EG)DB）。有人建议用后两种增塑剂替代前三种增塑剂，因为前三种增塑剂对环境有负面影响。D(PG)DB或D(EG)DB的降解导致溶液毒性显著增加，这与增塑剂不完全分解产生代谢物有关。D(PG)DB体系中的毒性代谢物被鉴定为二丙二醇单苯甲酸酯的异构体。提出了这种代谢物形成的途径。D(EG)DB被降解时观察到的代谢物与D(PG)DB体系相似，初步确定为二甘醇单苯甲酸酯。相比之下，红毛霉降解B(EH)A、DOP或DOTP的过程中，培养基中没有观察到代谢物，毒性也没有增加。总的来说，这些结果不支持使用D(PG)DB和D(EG)DB作为B(EH)A、DOP或DOTP的环境安全替代品。
The degradation of plasticizers by the yeast Rhodotorula rubra J‐96‐1 (American Type Culture Collection 9449) in the presence of glucose was studied. The plasticizers included the commonly used bis‐2‐ethylhexyl adipate (B(EH)A), dioctyl phthalate (DOP), and dioctyl terephthalate (DOTP), and the less commonly used dipropylene glycol dibenzoate (D(PG)DB) and diethylene glycol dibenzoate (D(EG)DB). The proposal had been made that the latter two plasticizers be used as alternatives to the first three, which have been associated with negative environmental impacts. The degradation of D(PG)DB or D(EG)DB led to a significant increase in solution toxicity, which was associated with the production of metabolites resulting from the incomplete breakdown of the plasticizers. The toxic metabolites in the D(PG)DB system were identified as isomers of dipropylene glycol monobenzoate. A pathway for the formation of this metabolite was proposed. The metabolite observed when D(EG)DB was being degraded was tentatively identified as diethylene glycol monobenzoate by analogy to the D(PG)DB system. In contrast, no metabolites were observable and toxicity did not increase in the media during the degradation of B(EH)A, DOP, or DOTP by R. rubra. Collectively, these results do not support the use of D(PG)DB and D(EG)DB as environmentally safe alternatives to B(EH)A, DOP, or DOTP.