Membrane-Active Action Mode of Polybia-CP, a Novel Antimicrobial Peptide Isolated from the Venom of Polybia paulista
Membrane-Active Action Mode of Polybia-CP, a Novel Antimicrobial Peptide Isolated from the Venom of Polybia paulista
复制标题
从Polybia paulista 毒液中分离的新型抗菌肽Polybia-CP 的膜活性作用模式
DOI：
10.1128/aac.05995-11
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发表时间：
2012-06-01
影响因子：
4.9
通讯作者：
Wang, Rui
中科院分区：
文献类型：
--
作者：
Wang, Kairong;Yan, Jiexi;Wang, Rui
摘要抗生素在医学、食品工业和农业中的广泛使用导致了多重耐药菌的频繁出现，这就迫切需要新的抗生素。抗菌肽（Ambioticpeptides，AMPs）在对抗多重耐药菌方面具有广阔的应用前景。抗菌肽Polybia-CP是从社会胡蜂Polybia保利斯塔的毒液中纯化得到的。本研究合成了Polybia-CP，并研究了其抑菌作用方式。我们的研究结果表明，polybia-CP对革兰氏阳性菌和革兰氏阴性菌都具有有效的抗菌活性。对真实的细菌膜和体外模型膜的研究结果表明，Polybia-CP具有膜活性，其作用靶是细菌膜。细菌很难对Polybia-CP产生耐药性，这可能为医学和食品及农业行业防御耐药细菌提供新的策略。
ABSTRACT The extensive use of antibiotics in medicine, the food industry, and agriculture has resulted in the frequent emergence of multidrug-resistant bacteria, which creates an urgent need for new antibiotics. It is now widely recognized that antimicrobial peptides (AMPs) could play a promising role in fighting multidrug-resistant bacteria. Antimicrobial peptide polybia-CP was purified from the venom of the social wasp Polybia paulista. In this study, we synthesized polybia-CP and studied its action mode of antibacterial activity. Our results revealed that polybia-CP has potent antibacterial activity against both Gram-positive and Gram-negative bacteria. The results from both the real bacterial membrane and the in vitro model membrane showed that polybia-CP is membrane active and that its action target is the membrane of bacteria. It is difficult for bacteria to develop resistance to polybia-CP, which may thus offer a new strategy for defending against resistant bacteria in medicine and the food and farming industries.