EFFECTS OF OPIOID-PEPTIDES AND NALOXONE ON NERVOUS-TISSUE IN CULTURE
EFFECTS OF OPIOID-PEPTIDES AND NALOXONE ON NERVOUS-TISSUE IN CULTURE
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DOI：
10.1016/0306-4522(87)90368-x
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发表时间：
1987-08-01
期刊：
影响因子：
3.3
通讯作者：
BESPALOVA, ZD
中科院分区：
文献类型：
--
作者：
ILYINSKY, OB;KOZLOVA, MV;BESPALOVA, ZD
研究表明，阿片肽刺激大鼠培养物中的神经组织生长，这表现为从外植体生长的神经突增加以及生长区中神经胶质细胞和成纤维细胞样细胞数量增加。阿片肽（[Leu]-和[Met]-脑啡肽，β-和γ-用大鼠交感神经节、背根神经节和脊髓的器官型培养，研究了内啡肽和某些合成的[Leu]-脑啡肽类似物对生长的影响。神经突生长，细胞成分，生长区的大小以及其形成的动力学进行了估计。测定背根神经节培养物中神经元存活的变化。用活培养物和固定制剂进行实验。在交感神经节的实验中，证明了以10-8 M至10-14 M的浓度摄取的肽发挥显著的生长促进作用。纳洛酮不能消除肽的作用，但在10-5 M至10-7 M时刺激生长。脊髓研究表明，纳洛酮（10-6 M）可增强对[Leu]-脑啡肽（10-9 M）的反应。在[leu]-脑啡肽类似物（10-9 M）的影响下，背根神经节神经元的存活率超过对照值约2至4倍。因此，阿片肽显示出对培养的神经组织发挥强烈的生长促进作用。这种影响是双重的：在神经元中，这些肽刺激神经突的生长和它们的存活，而在神经胶质细胞中，它们改变它们的迁移速率，并且可能改变它们的增殖速率。这表明，阿片肽，除了他们已经建立的功能，可能在神经组织的发育和再生中发挥作用。
It was shown that opioid peptides stimulate nervous tissue growth in culture in the rat, which manifests itself in augmented outgrowth of neurites from explants and in an increase in the number of glial and fibroblast-like cells in the growth zone. The effects of opioid peptides ([Leu]- and [Met]-enkephalins, .beta.- and .gamma.-endorphins and some synthetic analogues of [Leu]-enkephalin) on the growth of organotypic cultures of rat sympathetic and dorsal root ganglia and spinal cord were investigated. Neurite outgrowth, cell composition, and size of the growth zone as well as the dynamics of its formation were estimated. Changes in the survival of neurons in dorsal root ganglion cultures were determined. The experiments were performed with living cultures as well as with fixed preparations. In experiments with sympathetic ganglia, it was demonstrated that a significant growth-promoting effect is exerted by peptides taken at concentrations of 10-8 M to 10-14 M. Naloxone does not eliminate the effects of peptides, but stimulates the growth at 10-5 M to 10-7 M. Studies with spinal cord revealed that naloxone (10-6 M) enhances the response to [Leu]-enkephalin (10-9 M). The survival of dorsal root ganglion neurons under the influence of a [leu]-enkephalin analog (10-9 M) exceeds control values by approximately two to four times. Thus, opioid peptides were shown to exert a strong growth-promoting effect on nervous tissue in culture. This effect is dual: in neurons the peptides stimulate the outgrowth of neurites and their survival, while in glial cells they change the rate of their migration and, probably, their proliferation. It is suggested that opioid peptides, besides their already established functions, may play a role in the development and regeneration of nervous tissue.