Role of glutathione metabolism status in the definition of some cellular parameters and oxidative stress tolerance of Saccharomyces cerevisiae cells growing as biofilms
Role of glutathione metabolism status in the definition of some cellular parameters and oxidative stress tolerance of Saccharomyces cerevisiae cells growing as biofilms
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DOI：
10.1111/j.1567-1364.2008.00401.x
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发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Leroy, Pierre
中科院分区：
文献类型：
--
作者：
Gales, Gregoire;Penninckx, Michel;Leroy, Pierre
在删除与谷胱甘肽代谢和氧化应激相关基因的酵母突变体的帮助下，在生物膜和浮游细胞中比较了酿酒酵母对氧化应激（H2O2和Cd2+）的抵抗力。发现生物膜形成细胞主要处于细胞周期的 G1 阶段。这可能解释了它们对氧化应激的更高耐受性以及这些细胞在旧培养物中的年轻复制年龄。酿酒酵母的还原型谷胱甘肽状态受到生长期的影响，并且显然在作为生物膜生长的细胞的氧化应激耐受性中发挥着重要作用。
The resistance of Saccharomyces cerevisiae to oxidative stress (H2O2 and Cd2+) was compared in biofilms and planktonic cells, with the help of yeast mutants deleted of genes related to glutathione metabolism and oxidative stress. Biofilm-forming cells were found predominantly in the G1 stage of the cell cycle. This might explain their higher tolerance to oxidative stress and the young replicative age of these cells in an old culture. The reduced glutathione status of S. cerevisiae was affected by the growth phase and apparently plays an important role in oxidative stress tolerance in cells growing as a biofilm.