Spinal Cord Injury and Assays for Regeneration.
Spinal Cord Injury and Assays for Regeneration.
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脊髓损伤和再生测定。
DOI：
10.1007/978-1-0716-3401-1_14
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Mokalled,MayssaH
中科院分区：
文献类型：
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作者：
Burris,Brooke;Mokalled,MayssaH
由于其著名的再生能力，成年斑马鱼是一个首要的脊椎动物模型来询问先天脊髓再生机制。在脊髓完全横断后，斑马鱼在切断的组织中延伸神经胶质和轴突桥，在损伤近端再生神经元，并在损伤后8周内恢复游泳能力。在这里，我们描述了进行完全脊髓横断和评估再生过程中的功能和细胞恢复的方法。对于脊髓损伤，在脑干尾部4mm处进行完全横断。游泳耐力被量化为脊髓功能性修复的中心读数。在游泳耐力方面，斑马鱼受到不断增加的水流速度的影响，直到精疲力竭，并报道了精疲力竭的时间。为了评估细胞再生，进行组织学检查以分析病变上神经胶质和轴突桥接的程度。
Due to their renowned regenerative capacity, adult zebrafish are a premier vertebrate model to interrogate mechanisms of innate spinal cord regeneration. Following complete transection to their spinal cord, zebrafish extend glial and axonal bridges across severed tissue, regenerate neurons proximal to the lesion, and regain swim capacity within 8 weeks of injury. Here, we describe methods to perform complete spinal cord transections and to assess functional and cellular recovery during regeneration. For spinal cord injury, a complete transection is performed 4 mm caudal to the brainstem. Swim endurance is quantified as a central readout of functional spinal cord repair. For swim endurance, zebrafish are subjected to a constantly increasing water current velocity until exhaustion, and time at exhaustion is reported. To assess cellular regeneration, histological examination is performed to analyze the extents of glial and axonal bridging across the lesion.