Gene Expression of Analysis Identifies Over-expression of CXCL1, SPARC, SPP1, and SULF1 in Gastric Cancer
Gene Expression of Analysis Identifies Over-expression of CXCL1, SPARC, SPP1, and SULF1 in Gastric Cancer
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DOI：
10.1002/gcc.20715
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发表时间：
2010-01-01
影响因子：
3.7
通讯作者：
Monni, Outi
中科院分区：
文献类型：
--
作者：
Junnila, Siina;Kokkola, Arto;Monni, Outi
为了阐明与胃癌发生相关的基因表达特征，我们对46例芬兰人和20例日本人的胃组织进行了全基因组表达分析。芬兰和日本数据集之间的比较分析，确定了58个共同的基因，癌性和非肿瘤性胃组织之间的差异表达。这些基因中有26个在癌症中上调，32个下调。在这些基因中，64%的基因在另一个不相关的公开数据集中也有差异表达。采用实时荧光定量RT-PCR方法检测了82例胃组织中CXCL 1、SPP 1、SULF、CXCL 1、SULF的表达水平。该分析验证了来自微阵列分析的结果，因为与正常组织相比，这四种基因在癌组织中的表达显著更高（倍数变化3.4-8.9）。CXCL 1的过度表达也与生存率的提高呈正相关。总之，无论微阵列平台或患者人群如何，鉴定了58个基因的共同胃癌基因表达特征，包括CXCL 1，SPP 1，SULF。这些基因代表了胃癌的潜在生物标志物。(C)2009 Wiley-Liss，Inc.
To elucidate gene expression signatures associated with gastric carcinogenesis, we performed a genome-wide expression analysis of 46 Finnish and 20 Japanese gastric tissues. Comparative analysis between Finnish and Japanese datasets; identified 58 common genes that were differentially expressed between cancerous and non-neoplastic gastric tissues. Twenty-six of these genes were up-regulated in cancer and 32 down-regulated. Of these genes, 64% were also differentially expressed in another unrelated publicly available dataset. The expression levels of four of the up-regulated genes, CXCL1, SPARC, SPP1 and SULF were further analyzed in 82 gastric tissues using quantitative real-time RT-PCR. This analysis validated the results from the microarray analysis as the expression of these four genes was significantly higher in the cancerous tissue compared with the normal tissue (fold change 3.4-8.9). Over-expression of CXCL1 also positively correlated with improved survival. To conclude, irrespective of the microarray platform or patient population, a common gastric cancer gene expression signature of 58 genes, including CXCL1, SPARC, SPP1, and SULF was identified. These genes represent potential biomarkers for gastric cancer. (C) 2009 Wiley-Liss, Inc.