Rapid cytologic diagnosis of cytomegalovirus interstitial pneumonia on touch imprints from open-lung biopsy.
Rapid cytologic diagnosis of cytomegalovirus interstitial pneumonia on touch imprints from open-lung biopsy.
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通过开肺活检的接触印迹快速细胞学诊断巨细胞病毒间质性肺炎。
DOI：
10.1093/ajcp/77.1.90
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发表时间：
1982
影响因子：
3.5
通讯作者：
Meyers,JD
中科院分区：
文献类型：
--
作者：
Shulman,HM;Hackman,RC;Sale,GE;Meyers,JD
研究了瑞氏吉姆萨染色的触摸印记在骨髓移植受者的开肺活检中识别巨细胞病毒（CMV）的诊断效用。在 41 个连续活检标本中，18 个经培养证实具有 CMV，15 个在永久组织学切片上具有典型的核内和胞浆内包涵体。 15 个 CMV 活检标本中的 10 个的瑞氏吉姆萨染色触摸印记具有特征性增大的细胞，含有 20-75 个圆形至椭圆形胞浆内包涵体，而很少含有核内包涵体。赖特-吉姆萨染色印记 (10/15) 对 CMV 的诊断优于苏木精和伊红 (H & E) 染色接触印记 (5/15) 和 H & E 染色冰冻切片 (2/15)。两例巨细胞病毒具有巴氏染色印记，也显示出核内和胞浆内包涵体。这种检查开胸肺活检标本细胞学印记的简单技术将快速识别所有确诊 CMV 病例中 50% 以上，从而实现更早、可能更成功的抗病毒治疗。
The diagnostic utility of Wright-Giemsa-stained touch imprints for identifying cytomegalovirus (CMV) in. open-lung biopsies from marrow transplant recipients was studied. Of 41 consecutive biopsy specimens, 18 had culturally proven CMV,.and 15 had typical intranuclear and intracytoplasmic inclusion bodies on permanent histologic sections. Wright-Giemsa-stained touch imprints from ten of the 15 biopsy specimens with CMV had characteristically enlarged cells containing 20–75 round to oval intracytoplasmic inclusions while only rarely containing intranuclear inclusions. The diagnosis of CMV from Wright-Giemsa-stained imprints (10/15) was superior to hematoxylin and eosin (H & E)-stained touch imprints (5/15) and H & Estained frozen sections (2/15). Two cases of CMV had Papanicolaou-stained imprints that also demonstrated both intranuclear and intracytoplasmic inclusions. This simple technic of examining cytologic imprints from open-lung biopsy specimens will rapidly identify more than.50% of all confirmed cases of CMV, allowing earlier and possibly more successful antiviral therapy.