Alcohol oxidase- and formaldehyde dehydrogenase-based enzymatic methods for formaldehyde assay in fish food products
Alcohol oxidase- and formaldehyde dehydrogenase-based enzymatic methods for formaldehyde assay in fish food products
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DOI：
10.1016/j.foodchem.2010.12.146
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发表时间：
2011-07-15
期刊：
影响因子：
8.8
通讯作者：
Gonchar, Mykhailo
中科院分区：
文献类型：
--
作者：
Sibirny, Vladimir;Demkiv, Olha;Gonchar, Mykhailo
对于棘鱼来说，在内源性酶降解天然渗透保护剂三甲胺- n -氧化物的过程中，甲醛可以在组织中大量产生。本文介绍了利用从耐高温甲基营养酵母中分离的乙醇氧化酶（AOX）和甲醛脱氢酶（FdDH）测定鱼类食品中甲醛的两种酶法。基于aox的方法利用酶将水合形式的甲醛氧化成甲酸和过氧化氢的能力，在过氧化物酶催化的比色反应中进行监测。在基于fddh的方法中，在依赖甲醛的反应中产生的NADH还原过程中，由硝基四氮唑盐形成一种受监测的有色甲醛。结果表明，两种方法均适用于鱼制品中甲醛的测定。阐述了分析程序的最佳方案，并建立了两种酶法的分析参数。这两种方法都证明，一些鱼类产品（鳕鱼和鳕鱼）含有高浓度甲醛（高达100毫克/公斤湿重）。(C) 2011 Elsevier Ltd.版权所有。
For Gadoid fishes, formaldehyde can be generated in tissues in huge amounts during endogenous enzymatic degradation of natural osmoprotectant trimethylamine-N-oxide. This paper describes two enzymatic methods for assay of formaldehyde in fish food products using alcohol oxidase (AOX) and formaldehyde dehydrogenase (FdDH) isolated from the thermotolerant methylotrophic yeast Hansenula polymorpha. AOX-based method exploits an ability of the enzyme to oxidise a hydrated form of formaldehyde to formic acid and hydrogen peroxide monitored in peroxidase-catalysed colorimetric reaction. In FdDH-based method, a monitored coloured formazane is formed from nitrotetrazolium salt during reduction by NADH, produced in formaldehyde-dependent reaction. It was demonstrated an applicability of both methods for assay of formaldehyde in fish products. The optimal protocols for analysis procedures have been elaborated and analytical parameters of both enzymatic methods have been established. The both methods were demonstrated that some fish products (hake and cod) contain high formaldehyde concentrations (up to 100 mg/kg wet weight). (C) 2011 Elsevier Ltd. All rights reserved.