The chromosomal localization, expression pattern and polymorphism analysis of porcine FSCN1 gene differently expressed from LongSAGE library
The chromosomal localization, expression pattern and polymorphism analysis of porcine FSCN1 gene differently expressed from LongSAGE library
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LongSAGE文库差异表达猪FSCN1基因的染色体定位、表达模式及多态性分析
DOI：
10.1007/s11033-009-9742-9
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Li, K.
中科院分区：
文献类型：
--
作者：
Tang, Zh. L.;Zhang, X. J.;Li, K.
成束蛋白同源物1（FSCN 1）在细胞粘附、运动和细胞-细胞相互作用中具有确定的作用。我们的LongSAGE分析表明，FSCN 1可能在产前猪骨骼肌中差异表达。我们克隆了FSCN 1基因的基因组DNA和mRNA序列，并将其定位于SSC 3 p16-p17。FSCN 1基因在通城猪和长白猪骨骼肌发育过程中的表达存在差异，并表现出不同的表达模式。在通城猪中，FSCN 1在受孕后33天和65天（dpc）的表达相似，然后急剧增加，在90 dpc达到峰值。然而，在长白猪中，表达在33和65 dpc之间增加，在65 dpc达到峰值，此后下调。在65和90 dpc时，通城猪和长白猪的表达水平存在显著差异。在出生后的猪中，它仅在脑中强表达，而在骨骼肌和其他组织中弱表达。我们通过FSCN 1基因的基因组DNA初步鉴定出32个SNP。关联分析表明，6840（C/T）突变与上市体重年龄（AGE）（p = 0.0004）、出生至上市平均日增重（ADG 1）（p = 0.0002）和试验期平均日增重（ADG 2）（p < 0.0001）显著相关。本研究表明FSCN 1基因在胚胎期骨骼肌发育中起重要作用，是产肉性状的候选基因。
Fascin homologue 1 (FSCN1) has established roles in cell adhesion, motility, and cell-cell interactions. Our LongSAGE analysis suggested that FSCN1 was potentially differentially expressed in prenatal pig skeletal muscle. We have cloned the genomic DNA and mRNA sequence of FSCN1 gene and mapped it to SSC3p16-p17. The FSCN1 gene was differently expressed during prenatal skeletal muscle development and exhibited different expression pattern between Tongcheng and Landrace pigs. In Tongcheng pigs, FSCN1 expression was similar at 33 and 65 days post conception (dpc), and then sharply increased to a peak at 90 dpc. In Landrace pigs, however, expression increased between 33 and 65 dpc, peaked at 65 dpc, and was down-regulated thereafter. Significantly different expression levels between Tongcheng and Landrace were observed at 65 and 90 dpc. In postnatal pigs, it was strongly expressed only in the brain, but weakly in skeletal muscle and other tissues. We initially identified 32 SNPs through genomic DNA of FSCN1 gene. Association analysis suggested that the 6840(C/T) mutation was significantly associated with the age at market weight (AGE) (p = 0.0004), average day gain from birth to market (ADG1) (p = 0.0002), and average day gain at testing period (ADG2) (p < 0.0001). Our study suggested that FSCN1 gene plays an in prenatal skeletal muscle development and was a candidate gene for meat production trait.