Identification of drought-inducible genes and differentially expressed sequence tags in barley
Identification of drought-inducible genes and differentially expressed sequence tags in barley
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DOI：
10.1007/s00122-004-1755-0
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发表时间：
2004-11-01
影响因子：
5.4
通讯作者：
Sorrells, ME
中科院分区：
文献类型：
--
作者：
Diab, AA;Teulat-Merah, B;Sorrells, ME
干旱限制了世界一些地区的谷物产量，植物水分状况在耐旱性方面起着重要作用。为了研究和了解大麦耐旱性的遗传和生理基础，在167个F-8重组自交系中定位了干旱响应的差异表达序列标签（dsts）和候选基因，这些自交系是由耐旱性的“Tadmor”和仅适应特定干旱环境的“Er/Apm”杂交而来。从先前构建的两个干旱胁迫大麦（Hordeum vulgare L., var. Tokak）植物cDNA文库和12个候选基因中提取100个测序探针进行多态性分析，并将33个位点添加到先前发表的图谱中。利用复合区间作图技术，鉴定了水稻叶片相对含水量、叶片渗透势、满膨时渗透势、水溶性碳水化合物浓度、渗透调节和碳同位素识别等与抗旱性相关的QTL位点。两种水处理评价性状和两种水处理计算性状共检测到68个检测限大于或等于2.5的qtl。每个性状的qtl数量从1到12不等，表明基因组包含影响不同性状的多个基因。2个候选基因和10个差异表达序列与抗旱性状qtl相关。
Drought limits cereal yields in several regions of the world and plant water status plays an important role in tolerance to drought. To investigate and understand the genetic and physiological basis of drought tolerance in barley, differentially expressed sequence tags (dESTs) and candidate genes for the drought response were mapped in a population of 167 F-8 recombinant inbred lines derived from a cross between "Tadmor" (drought tolerant) and "Er/Apm" (adapted only to specific dry environments). One hundred sequenced probes from two cDNA libraries previously constructed from drought-stressed barley (Hordeum vulgare L., var. Tokak) plants and 12 candidate genes were surveyed for polymorphism, and 33 loci were added to a previously published map. Composite interval mapping was used to identify quantitative trait loci (QTL) associated with drought tolerance including leaf relative water content, leaf osmotic potential, osmotic potential at full turgor, water-soluble carbohydrate concentration, osmotic adjustment, and carbon isotope discrimination. A total of 68 QTLs with a limit of detection score greater than or equal to2.5 were detected for the traits evaluated under two water treatments and the two traits calculated from both treatments. The number of QTLs identified for each trait varied from one to 12, indicating that the genome contains multiple genes affecting different traits. Two candidate genes and ten differentially expressed sequences were associated with QTLs for drought tolerance traits.