Immunohistochemical study of the upper surface layer in rat mandibular condylar cartilage.
Immunohistochemical study of the upper surface layer in rat mandibular condylar cartilage.
复制标题
大鼠下颌髁软骨上表面层的免疫组织化学研究。
DOI：
10.14670/hh-19.29
复制
发表时间：
2004
影响因子：
2
通讯作者：
Shinichi Ohno
中科院分区：
文献类型：
--
作者：
Z. Zea;K. Ohtsuki;M. Ohnishi;Shinichi Ohno
用免疫组织化学方法观察了透明质酸和纤维连接蛋白在大鼠髁突软骨上表面层的分布。通过关节盘髁突的制备，成功地保留了它们的精细结构。将多聚甲醛固定的软骨组织在低温恒温器中切割，并使用链霉亲和素-过氧化物酶和间接免疫荧光方法分析冷冻切片。采用免疫胶体金法结合常规制备方法和快速冷冻法进行超微结构分析。透明质酸结合蛋白和抗纤维连接蛋白抗体分别用于定位透明质酸和纤维连接蛋白在下颌髁突软骨中。一些冷冻切片在这样的标记之前用透明质酸酶和软骨素酶预处理。上表面层由双层结构组成。一个与软骨基质表面相邻，纤维连接蛋白阳性。透明质酸位于纤连蛋白层上。因此，体内的透明质酸与软骨基质中的纤维连接蛋白结合，对下颌关节运动起到润滑作用。
Both hyaluronic acid and fibronectin localizations were examined in the upper surface layer of rat mandibular condylar cartilages by immunohistochemical techniques. Their delicate structure was successfully preserved by preparation procedures of joint condyles with disks. Paraformaldehyde-fixed cartilaginous tissues were cut in a cryostat, and cryosections were analyzed using streptavidin-peroxidase and indirect immunofluorescence methods. Another immunogold method with conventional preparation procedures and a quick-freezing method was performed for their ultrastructural analyses. Both hyaluronic acid-binding protein and anti-fibronectin antibody were used to localize hyaluronic acid and fibronectin in the mandibular condylar cartilage, respectively. Some cryosections were pre-treated with hyaluronidase and chondroitinase before such labeling. The upper surface layer was composed of double laminar structures. One bordered with the cartilage matriceal surface, which was positive for fibronectin. The hyaluronic acid was localized over the fibronectin layer. Therefore, the hyaluronic acid in vivo was bound with fibronectin in the cartilaginous matrix, performing lubrication for the mandibular joint movement.