OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models
OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models
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DOI：
10.3748/wjg.14.4800
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发表时间：
2008-08-14
影响因子：
4.3
通讯作者：
Xu, Ling
中科院分区：
文献类型：
--
作者：
Shi, Jun;Wei, Pin-Kang;Xu, Ling
目的：利用OB胶膏技术建立裸鼠人胃癌原位移植模型。方法：采用OB胶膏技术，将SGC-7901和MKN-45人胃癌细胞株植入裸鼠胃壁，建立正位移植模型。观察两种模型的生物学特性、移植肿瘤生长情况、移植成功率和自身转移率。结果：两种模型原位移植成功率分别为94.20%和96%。肝转移、肺转移、腹膜转移、淋巴细胞转移和脾转移的发生率分别为42.13%和94.20%、48.43%和57.97%、30.83%和36.96%、67.30%和84.06%、59.75%和10.53%。腹水发生率分别为47.80%和36.96%。结论：OB胶膏技术简便易行。利用该技术建立的裸鼠人胃癌原位移植模型的生物学行为与人胃癌生长转移的自然过程相似，可作为肿瘤增殖转移实验研究的理想模型。(C) 2008 WJG出版社。版权所有。
AIM: To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique.METHODS: Using OB glue paste technique, orthtopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed.RESULTS: The success rates of orthotopic transplantation of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%.CONCLUSION: OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors. (C) 2008 The WJG Press. All rights reserved.