Differential persistence among genomovars of the Burkholderia cepacia complex in a murine model of pulmonary infection
Differential persistence among genomovars of the Burkholderia cepacia complex in a murine model of pulmonary infection
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DOI：
10.1128/iai.70.5.2715-2720.2002
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发表时间：
2002-05-01
影响因子：
3.1
通讯作者：
Speert, DP
中科院分区：
文献类型：
--
作者：
Chu, KK;Davidson, DJ;Speert, DP
感染洋葱伯克霍尔德氏菌复合体不同基因组的囊性纤维化患者可以经历不同的临床结果。为了确定可能导致这些差异的基因组特异性决定因素，我们建立了BALB/c小鼠肺部感染的模型。在用1.6×10(4)细菌鼻腔攻击之前，给小鼠注射环磷酰胺，使小鼠白细胞减少。6个基因变异体II菌株中有5个在肺中保持稳定数量，直到第16天毒性最小，而7个基因变异体III菌株中0个持续存在，但导致了不同的毒性。我们已经建立了洋葱假单胞菌感染的慢性肺部模型，该模型揭示了不同基因组在临床感染结果方面的差异。
Cystic fibrosis patients infected with strains from different genomovars of the Burkholderia cepacia complex can experience diverse clinical outcomes. To identify genomovar-specific determinants that might be responsible for these differences, we developed a pulmonary model of infection in BALB/c mice. Mice were rendered leukopenic by administration of cyclophosphamide prior to intranasal challenge with 1.6 X 10(4) bacteria. Five of six genomovar II strains persisted at stable numbers in the lungs until day 16 with minimal toxicity, whereas zero of seven genomovar III strains persisted but resulted in variable toxicity. We have developed a chronic pulmonary model of B. cepacia infection which reveals differences among genomovars in terms of clinical infection outcome.