Effects of Ultraviolet (UV) Radiation on Outdoor- and Indoor-Cultured Prorocentrum lima, a Toxic Benthic Dinoflagellate
Effects of Ultraviolet (UV) Radiation on Outdoor- and Indoor-Cultured Prorocentrum lima, a Toxic Benthic Dinoflagellate
复制标题
紫外线 (UV) 辐射对室外和室内培养的有毒底栖甲藻原甲藻的影响
DOI：
10.1007/s11802-021-4560-3
复制
发表时间：
2021-06-01
影响因子：
1.6
通讯作者：
Lu Songhui
中科院分区：
文献类型：
--
作者：
Chen Heng;Huang Kaixuan;Lu Songhui
研究了不同类型的紫外线（UVR，波长=280-400 nm）和光照强度对在室外培养16 d和室内培养18 d的利马原甲藻（Prorocentrum lima）细胞生长、色素组成、紫外吸收物质（UVACs）和叶绿素a（Chl a）荧光的影响。在室外实验中，当光照强度< 12%时，UVA辐射（320-400 nm）促进了该甲藻的生长，当光照强度> 12%时，生长速率下降。暴露于UVB辐射（280- 320 nm）减轻UVA的负面影响。在室内实验中，UVA和低剂量的UVB促进生长速率。低剂量的UVB与UVA照射相比，增加了叶绿素a和光保护色素的含量。两个实验的结果表明，UVB是UVAC合成的主要信号。高剂量UVB暴露加速生长速率时，UVAC含量保持在高水平，这表明后者在UVR损伤保护中起着关键作用。此外，在培养16天后，UVB暴露增强了修复率。这项研究证实了UVA和UVB对P. lima生长的积极影响，后者增强了该物种的光保护和恢复途径。
The effects of different types of ultraviolet (UV) radiation (UVR, wavelength=280-400 nm) and light intensities on cell growth, pigment composition, UV-absorbing compounds (UVACs), and chlorophyll a (Chl a) fluorescence were studied in dinoflagellate Prorocentrum lima cultured outdoors for 16 days and indoors for 18 days. In the outdoor experiment, UVA radiation (320-400 nm) increased the growth rate of this dinoflagellate when solar light intensities were < 12%; decreased growth rates were observed when intensities were> 12%. Exposure to UVB radiation (280-320nm) alleviated the negative effects of UVA. In the indoor experiment, UVA and low doses of UVB enhanced growth rates. Addition of low doses of UVB to UVA exposure resulted in higher contents of Chl a and photoprotective pigments compared with UVA exposure only. The results of both experiments showed that UVB is the primary signal of UVAC synthesis. High-dose UVB exposure accelerated growth rates when UVAC contents were maintained at high levels, suggesting that the latter plays a key role in UVR damage protection. Furthermore, the repair rate was enhanced by UVB exposure after 16 days of culture. This study confirms the positive effects of UVA and UVB on the growth of P. lima, with the latter enhancing the photoprotective and recovery pathways of the species.