Citric acid production by Yarrowia lipolytica cultivated on olive-mill wastewater-based media
Citric acid production by Yarrowia lipolytica cultivated on olive-mill wastewater-based media
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DOI：
10.1016/j.biortech.2007.05.005
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发表时间：
2008-05-01
影响因子：
11.4
通讯作者：
Aggelis, George
中科院分区：
文献类型：
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作者：
Papamkolaou, Seraphim;Galiotou-Panayotou, Maria;Aggelis, George
在橄榄磨坊废水（O.M.W.）上培养的解脂耶氏酵母ACA-DC 50109-基于培养基，富含商业-工业葡萄糖，呈现出有效的细胞生长。生长参数不受O.M.Ws在生长培养基中的存在的影响，在稀释的O.M.Ws富含高葡萄糖量（初始糖浓度，65 g l（-1）），产生显着量的总柠檬酸（28.9 g l（-1））。我的天基础培养基对柠檬酸的生产具有显著的刺激作用，因为当与从不添加O.M.W.的试验获得的相应参数相比时，每单位消耗的糖产生的柠檬酸的最终柠檬酸浓度和转化产率都更高。OMW中菌株的适应-基于培养基有利于细胞不饱和脂肪酸（主要是油酸和棕榈油酸）的生物合成。此外，生长培养基中酚类化合物的不可忽略的降低[高达15%（wt/wt）]，植物毒性的轻微降低，以及O.M.W.观察了这些结果表明有机微囊藻在柠檬酸发酵过程中的应用潜力。(c)2007爱思唯尔有限公司保留所有权利。
Yarrowia lipolytica ACA-DC 50109 cultivated on olive-mill wastewater (O.M.W.)-based media, enriched with commercial-industrial glucose, presented an efficient cell growth. Parameters of growth were unaffected by the presence of O.M.Ws in the growth medium, In diluted O.M.Ws enriched with high glucose amounts (initial sugar concentration, 65 g l(-1)), a notable quantity of total citric acid was produced (28.9 g l(-1)). O.M.W.-based media had a noteworthy stimulating effect on the production of citric acid, since both final citric acid concentration and conversion yield of citric acid produced per unit of sugar consumed were higher when compared with the respective parameters obtained from trials without added O.M.W. Adaptation of the strain in O.M.W.-based media favoured the biosynthesis of cellular unsaturated fatty acids (principally of oleic and palmitoleic acids). Additionally, a non-negligible decrease of the phenolic compounds in the growth medium [up to 15% (wt/wt)], a slight decrease of the phyto-toxicity, and a remarkable decolourisation of the O.M.W. were observed. All these results suggest the potentiality of O.M.Ws utilisation in the fermentation process of citric acid production. (c) 2007 Elsevier Ltd. All rights reserved.